12
13
Change the clock (optional)
Press
Set date and time
Use the up and down arrows to set the time and date
manually. Then press
OK
.
Notes:
• Time is displayed in hours : minutes : seconds and the date
is displayed in month/day/year.
• Check
DST
to automatically update clock to daylight saving
time.
Cancel
OK
Time zone: (UTC -08:00) Pacific Time (US & Canada)
Time
2 : :
14 36 PM
Date
04
/ /
09 2020
DST
Running the BioXp™ system
Once you’ve ordered and received your BioXp™ application project, running the BioXp™ system is as easy as loading the deck
and pressing
Start
.
Receiving a BioXp™ application project
From building gene fragments and clones to library construction, BioXp™ applications come with everything you need to
complete projects. The project components are made up of modules.
Gene
fragments
Clones
amplification
Variant
libraries
Module A
(+4 °C)
Oligo Vault™ plate
Module A
(+4 °C)
Recovery plate
Module A
(+4 °C)
DNA purification strip
Module A
(+4 °C)
Custom vector strip
—
—
Module B
(−20 °C)
DNA assembly reagent plate
Module C
(−20 °C)
Gibson Assembly® cloning strip
—
—
Module D
(−20 °C)
RapidAMP™ strip
—
—
—
Preparing the BioXp™ system for a run
1. Load tips by aligning the tip tray notch with the
upper left corner
of each tip tray retainer.
a. Load 3 × 50 μL tips
b. Load 1 × 200 μL tips
2. Add a minimum of 12 mL freshly prepared 70% ethanol to the reusable
ethanol reservoir
.
a. Load
ethanol reservoir
in the right-most reservoir retainer position of the instrument deck.
Note: Do not discard the ethanol reservoir after the run; keep for future use.
Loading the BioXp™ deck
Refer to
BioXp™ gene fragments and variant libraries — Loading map and checklist
or the
BioXp™ cloning projects — Loading
map and checklist
document for project-specific loading instructions.
Reagent strips
BioXp™ gene fragments and variant libraries
BioXp™ c amplification projects
. . . .
BioX
p™ DNA
assembly plate
P/N: BX1230
-XX
Lot: 123
MFG date: 09/01/2020
BioXp™ system
Oligo Vault™
OLIGO VAULT™ PLATE
RECOVERY PLATE
50 µL TIPS
50 µL TIPS
50 µL TIPS
200 µL TIPS
DNA ASSEMBLY REAGENT PLATE
THERMAL COVER
ETHANOL RESERVOIR
REAGENT STRIPS
. . . .
BioX
p™ DNA
assembly plate
P/N: BX1230
-XX
Lot: 123
MFG date: 09/01/2020
BioXp™ 3200
Oligo vault
OLIGO VAULT™ PLATE
RECOVERY PLATE
50 µL TIPS
50 µL TIPS
50 µL TIPS
200 µL TIPS
DNA ASSEMBLY REAGENT PLATE
THERMAL COVER
ETHANOL RESERVOIR
REAGENT STRIPS
Refer to
BioXp™ cloning projects — Loading map and checklist
document for preparing custom vector strip.
Gene fragments
and variant libraries
Cloning
Cloning and
RapidAMP™ amplification
. . . .
1
Pinholes
2 3 4
. . . .
1
Pinholes
2 3 4
. . . .
1
Pinholes
2 3 4
Position 1 — black
DNA
purification strip
Position 2 — blue
RapidAMP™ strip
Position 3 — purple
Gibson Assembly®
cloning strip
Position 4 — red
custom
BioXp™ vector strip