Cellink BIO X6 User Manual Download Page 101

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BIO X6 User Manual v. 1.0

101

5.5 Gcode preview mode

The Gcode Print preview option allows you to preview the travel moves of each line of your

code.

1. Tap the

Right end arrow

to manually move forward to the next line of code.

2. Tap the

Left end arrow

to manually move to previous line of code and undo the

travel move.

3. A line-by-line preview of each travel move, showing the nozzle in the same color as

the selected printhead.

Figure 74 Overview of the preview mode, part 1

Summary of Contents for BIO X6

Page 1: ...BIO X6 User Manual v 1 0 1 BIO X6 User Manual...

Page 2: ...lity the BIO X6 will take your research to the next level With interchangeable Intelligent Printheads Clean Chamber technology print chamber sterilization a temperature controlled printbed an improved...

Page 3: ...erview 15 2 2 Technical specifications 17 3 Getting started 20 3 1 Unpacking the BIO X6 20 3 2 Contents of the box 22 3 3 Setting up the BIO X6 24 3 4 Starting the BIO X6 for the first time 25 3 5 Cal...

Page 4: ...ocrosslinking Model 74 4 5 Bioprinting droplets 75 4 5 1 Print surface 76 4 5 2 Droplet 77 4 5 3 Printhead settings 78 4 5 4 Printer settings 80 4 5 5 Summary 82 4 5 6 Calibration and Auto Bed Levelli...

Page 5: ...isites 123 8 2 Opening and adding comments to print report 124 8 3 Information included in the print report 125 8 4 Opening previous print reports 129 9 Coaxial printing 131 9 1 Coaxial set up 131 9 2...

Page 6: ...2 3 Reservation of rights 156 11 2 4 Updates or revisions 156 11 2 5 Trademarks 156 11 2 6 Third party materials 157 11 2 7 Digital content and data 157 11 2 8 Limitation of liability 158 11 2 9 Restr...

Page 7: ...BIO X6 User Manual v 1 0 7 12 3 CELLINK Thermoplastic Printhead 172 12 3 1 Getting started 172 12 3 2 Printhead overview 173 12 3 3 Assembling the Thermoplastic Printhead 174...

Page 8: ...BIO X6 User Manual v 1 0 8 01 Safety...

Page 9: ...ol indicates a heated surface The BIO X6 has heated surfaces that may cause personal injury or equipment damage if not treated with care A physical symbol labels these surfaces on the BIO X6 and the s...

Page 10: ...luding changing the filter The BIO X6 uses UV light for sterilization and curing Never look directly at UV light Never expose skin to UV light Eye or skin exposure can result in serious personal injur...

Page 11: ...BIO X6 to a grounded socket and use the voltage described in the specifications Improper electrical handling can cause equipment damage and personal injury 1 4 Protective equipment Always wear gloves...

Page 12: ...mmable vapors have been ventilated 1 6 Electrostatic discharge ESD protection The BIO X6 print chamber is ESD sensitive Follow the instructions below to ensure that you do not damage the BIO X6 or its...

Page 13: ...tegration or fitness for a particular purpose You assume responsibility for selecting the solution to achieve your intended results and for the installation of use of and results obtained from the equ...

Page 14: ...BIO X6 User Manual v 1 0 14 02 Specifications...

Page 15: ...Manual v 1 0 15 2 Specifications 2 1 Product overview Figure 1 Front view of BIO X6 1 Clean Chamber unit 2 Printbox printhead mount 1 6 3 Photocuring LED 1 4 4 Printbed 5 UV protective door 6 Docking...

Page 16: ...BIO X6 User Manual v 1 0 16 Figure 2 Back view of BIO X6 1 Power switch 2 Fuse 3 Power socket 4 Pneumatic port 5 USB port 6 Ethernet port...

Page 17: ...t weight 47 4 kg 104 5lb Build volume L x W x H 128 x 90 x 85 mm Filter class HEPA 14 UV sterilization UV C 275 nm 2W output UV curing modules 365 nm 405 nm 475 nm 520 nm No of printhead slots 6 Calib...

Page 18: ...est temperature 17 C below chamber temperature Printhead temperature range 4 250 C dependent on printhead type Pneumatic printhead 30 65 C Temperature controlled Printhead 4 65 C lowest temperature 17...

Page 19: ...BIO X6 User Manual v 1 0 19 03 Getting started...

Page 20: ...e underside The BIO X6 is heavy approximately 50 kg 110 lbs Never attempt to lift it alone Always use a minimum of two people to unpack and move the BIO X6 Follow these steps to unpack the BIO X6 1 Cl...

Page 21: ...erview of equipment included with the BIO X6 7 Remove the docking station and the tablet from the compartment behind the BIO X6 8 With the assistance of a second person lift the BIO X6 from the unders...

Page 22: ...r and caps 1x non sterile high precision conical nozzles Blue 22G 50 pcs 1x non sterile high precision conical nozzles Red GA 25 50 pcs 1x non sterile high precision conical nozzles Clear GA 27 50 pcs...

Page 23: ...x Heat resistant tubing 1x Plunger removal with plunger x Thermoplastic wrench x Thermoplastic user manual Box Temperature controlled contains x Temperature controlled PH x Nozzle insulator x Steel ti...

Page 24: ...6 in a room with a temperature between 18 C and 22 C Under normal operating conditions the chamber temperature will rise between 2 C and 3 C and the temperature on the standard pneumatic printheads wi...

Page 25: ...d close door temperature status chamber lights clean chamber fan and sterilize chamber 4 New Print Protocol In this section you find the Model print Droplet print and Draw Print modes 5 Open close doo...

Page 26: ...v 1 0 26 15 Recent print protocols This section shows shortcuts to the last 18 protocols Protocols are saved with the file extension biox 16 Open print protocols Tap to access all biox protocols stor...

Page 27: ...ed 1 Tap Printing utilities Machine Calibration Perform auto calibration trimming 2 Attach all 6 pneumatic printheads Use the same kind of nozzle needle for all printheads 3 Attach the surface probe a...

Page 28: ...e only time you need to repeat it is when you upgrade or downgrade the firmware HeartOS If you are working with a specific nozzle needle gauge and the autocalibration trimming has been performed using...

Page 29: ...BIO X6 User Manual v 1 0 29 04 Bioprinting...

Page 30: ...ific printhead manuals for instructions on how to mount a cartridge Mounting a printhead in the BIO X6 1 Move the printhead straight down toward the printhead holder 2 Press it until you feel the magn...

Page 31: ...ligent printhead connector This connector connects the intelligent printhead to the BIO X6 2 Magnet The magnets hold the printhead in place 3 Pressure connector The pressure connector delivers compres...

Page 32: ...ng loaded arms to securely fasten the print surface on the printbed according to the illustration below Turn the arms to the sides when using a well plate Ensure the outer dimensions of the well plate...

Page 33: ...UV sterilization procedure before each bioprinting process to minimize the risk of contamination 1 Close the door of the chamber The UV sterilization process does not start if the door is open 2 To be...

Page 34: ...ting utilities menu On the home screen tap to show the left side menu Figure 10 Printing utilities menu on the home screen Printing Utilities Tap to access the Printing Utilities menu with advanced se...

Page 35: ...the Connect tab 1 Search for bioprinter on network Tap to search for a bioprinter connected to the same Wi Fi network as the tablet computer you are using 2 Bioprinters found on network Select the bi...

Page 36: ...es for experimental purposes All the changes to the printer settings done here are applied immediately to the BIO X6 Figure 12 Overview of the Printer tab 1 Sensors Display output data for customer su...

Page 37: ...ith photosensitive materials 3 Enable Clean Chamber fan Tap to turn the Clean Chamber fan on or off 4 Sterilize chamber Tap to initiate the Sterilize chamber protocol 5 Printbed temperature control Ta...

Page 38: ...The selected toolhead is highlighted in blue and its settings are displayed below The displayed settings may vary depending on the printhead or tool connected 2 Extrude Tap and hold to test the bioin...

Page 39: ...BIO X6 User Manual v 1 0 39 6 Flow delay Tap to set the flow delay 7 External pressure pump Tap to indicate if an external pressure pump is connected to the BIO X 6...

Page 40: ...e printhead Use the arrows to move the toolhead on the x and y axes Available step sizes are 5 1 0 1 and 0 01 mm 3 Move printbed Use the arrows to move the printbed on the z axis Available step sizes...

Page 41: ...BIO X6 User Manual v 1 0 41 The calibration is stored and remembered to perform automatic calibration in the Bioprint menu...

Page 42: ...e a photo of the live stream from the Camera tool 3 Move printbed Use the arrows to move the printbed on the z axis Available step sizes are 5 1 and 0 1 mm 4 Move printhead Use the arrows to move the...

Page 43: ...photocrosslinked and select the distance from the surface up down left right in increments of 5 1 or 0 1 mm 3 Light source Tap to select the light source for photocrosslinking You can choose one of th...

Page 44: ...SB flash drive into the BIO X6 In the Utilities menu go to Update Printer to access the update 1 Find updates at Use this link to download the latest software version to your tablet or computer 2 Choo...

Page 45: ...4 3 9 About tab Use this tab to view contact information for customer support the software version and the printer and software licenses 1 License Tap to show the printer and software license Figure 1...

Page 46: ...6 4 4 Bioprinting from a 3D model On the home screen tap Model to start a new bioprinting process using a 3D model The BIO X6 guides you through the process of setting up your bioprint Figure 20 Model...

Page 47: ...elect a vendor for the well plate you are using This feature enables the BIO X6 to know the precise dimensions of the well plate which may vary slightly depending on the vendor 1 Corning Costar 2 Corn...

Page 48: ...BIO X6 User Manual v 1 0 48 Figure 22 Custom well plate option...

Page 49: ...ts Figure 23 Open file option in the Model step 1 Insert a USB flash drive containing your 3D model and any custom profiles you would like to use into any of the USB ports on the back of the tablet do...

Page 50: ...ed here Tap the left button to transform your 3D model by rotating scaling and moving it Tap the trash bin to delete the selected file Any modifications can be reset or previewed in the center of any...

Page 51: ...choose between different options rotate drag zoom in and zoom out This option helps you visualize with more detail the models to print and check for issues on the model or with the slice 7 Preview The...

Page 52: ...ioprint material Modify to ensure sufficient adhesion of the first layer to the print surface 3 Infill pattern Tap to select infill pattern see Infill screenshot below 4 Infill density Indicate how de...

Page 53: ...erase a layer group 10 Create new layer group Tap to create a new layer group 11 Generate support structure Tap to automatically generate a support structure for your construct You will need to design...

Page 54: ...esulting in solid walls between the neighbouring pores with good compressive strength This pattern is most applicable for tissues like bone and cartilage This pattern can also generate constructs that...

Page 55: ...the structure is filled in Note that there is minimal crossing of filaments which might be detrimental for the structural integrity of the construct 6 Gyroid The gyroid pattern consists of wave like p...

Page 56: ...ess Tap on each printhead to set it up with the information in the two boxes below 3 9 3 Printhead type Tap to select the type of printhead you are using Different settings appear below depending on w...

Page 57: ...ect the speed of the printhead 8 Temperature Enable and set printhead temperature 9 Flow delay Set to compensate for delayed and excess extrusion 10 External pressure pump Tap to indicate if an extern...

Page 58: ...se this option it will overwrite the speed which user select from UI during setup the printer uses the print speed which is written already in Gcode 2 Enable Clean Chamber fan Tap to enable or disable...

Page 59: ...6 Printbed temperature control Tap to enable or disable temperature control on the printbed If disabled the printbed will be kept at room temperature 7 Travel settings Tap to change the speed of the t...

Page 60: ...ttings 3 Tool 2 Displays an illustration of the tool 2 and shows its settings 4 Tool 3 Displays an illustration of the tool 3 and shows its settings 5 Tool 4 Displays an illustration of the tool 4 and...

Page 61: ...settings 13 Save protocol Tap to save the current printing protocol with the BIOX file extension 14 Export gcode Tap to export the current printing protocol with the gcode file extension 15 Connection...

Page 62: ...s already defined 1 Calibrate printhead Tap to calibrate the printheads and the print bed 2 Adjust Parameters 3 Close Door Tap to close the door if the door is open 4 Open Door Tap to open the door if...

Page 63: ...evelling compensation It uses a matrix to compensate for tilt in the bed or print surface It is recommended to perform ABL when printing large models or when using well plates as the printing surface...

Page 64: ...ace Home the printer axes and return to absolute zero position 2 Done Tap to proceed with the bioprinting process This button will only be enabled once the calibration has been successfully completed...

Page 65: ...6 Surface icon Tap to review and edit the surface settings 7 Camera icon Tap to review and edit the camera settings This option is only available the Camera tool is connected to the BIO X6 8 Start pri...

Page 66: ...ro initials of each of the coordinate axes values the user must calibrate the heads to the surface with 0 0 distance depending on the surface used The printer runs a homing cycle to get the 0 values i...

Page 67: ...BIO X6 User Manual v 1 0 67 Petri dish Figure 40 Manual calibration Petri dish Well plate Figure 41 Manual calibration well plate Glass slide...

Page 68: ...BIO X6 User Manual v 1 0 68 Figure 42 Manual calibration glass slide...

Page 69: ...en you are done tap Save calibration You can repeat the same procedure to calibrate all the printheads in your system by using the same point on the selected surface as a reference Note Manual calibra...

Page 70: ...BIO X6 User Manual v 1 0 70 Figure 45 Overview of the manual calibration of each tool When you have calibrated all the toolheads tap Done and Start print Figure 46 Completing the manual calibration...

Page 71: ...ped Doing so risks aborting the process and compromising your cells 1 Printhead icon Allows you to monitor and change the settings of the printheads used in the bioprinting process 2 Surface icon Allo...

Page 72: ...n be used to print the same model by using the same parameters and print surface as the previous print 9 Manual photocrosslinking Tap to perform manual photocrosslinking on your final constructs You c...

Page 73: ...BIO X6 User Manual v 1 0 73 12 Abort Tap to cancel the print 13 Restart Tap to restart the print Figure 49 Printing model continued...

Page 74: ...slinked and select the distance from the surface up down left right and adjust it for precision by using 5 1 or 0 1 mm steps 3 Light source Tap to select the light source for photocrosslinking You can...

Page 75: ...5 4 5 Bioprinting droplets On the home screen tap Droplet to start to start bioprinting droplets without a Gcode The BIO X6 guides you through the process of setting up your bioprint Figure 51 Droplet...

Page 76: ...which can slightly vary depending on the vendor The current supported vendors are Corning Costar Corning Falcon Thermo Fisher and VWR 3 Custom well plate Tap to create a custom well plate 4 Preview A...

Page 77: ...thead you want to use Different settings will appear below depending on what type of printhead you select Droplet printing is compatible with Standard Pneumatic Temperature controlled and Syringe Pump...

Page 78: ...ith Standard Pneumatic Temperature controlled and Syringe Pump printheads 3 Nozzle type Tap to select the nozzle type No matter if the user is using needles or nozzles user is allowed to choose the no...

Page 79: ...nal air compressor and between 0 700 kPa with an external air compressor Extrusion pressure depends mainly on the viscosity and rheological properties of the material to be printed 6 Extrusion time Ta...

Page 80: ...e applied once the bioprinting process has started 1 Tap to use GCode speed during print it will override the speed which user select during setup 2 Enable Clean Chamber fan Tap to enable or disable t...

Page 81: ...Printbed temperature control Tap to enable or disable temperature control on the printbed If disabled the printbed will be kept at room temperature 7 Travel settings Tap to change the speed of the tr...

Page 82: ...ts in blue the step you are currently in 2 Tool 1 Displays an illustration of the tool 1 and shows its settings 3 Tool 2 Displays an illustration of the tool 2 and shows its settings 4 Tool 3 Displays...

Page 83: ...for the printer Tap to edit settings 11 Save protocol Tap to save the current printing protocol as a file with the BIOX extension 12 Export gcode Tap to save que current printing protocol as a file w...

Page 84: ...ibrate Printheads Tap to calibrate the printheads and the print bed 2 Adjust Parameters Tap to open the right side of settings for the print 3 Open Door Tap to open the door if the door is closed 4 St...

Page 85: ...ABL probes the bed at three points to enable bed levelling compensation It uses a matrix to compensate for tilt in the bed or print surface It is recommended to perform ABL when printing large models...

Page 86: ...alibration you must first attach the surface probe to the printhead connector body and the metal reflectors to the printbed Then tap Continue to proceed Figure 59 Automatic calibration dialog 1 Drople...

Page 87: ...rinter settings 6 Surface icon Tap to review and edit the surface settings 7 Camera icon Tap to review and edit the camera settings This option is only available when the Camera tool is connected to t...

Page 88: ...printing process 2 Surface icon Allows you to monitor the settings of the print surface 3 Printer icon Allows you to monitor and change the settings of the printer 4 Camera icon Allows you review and...

Page 89: ...rint surface as the previous print 9 Manual Photocrosslinking Tap to perform manual photocrosslinking on your final constructs You can select wells the wavelength the distance from the printbed and th...

Page 90: ...cision by using 5 1 or 0 1 mm steps 3 Light source Tap to select the light source for photocrosslinking You can choose one of the UV curing LEDs or the Photocrosslinking tool 4 Intensity Tap to change...

Page 91: ...ow these parameters can influence your print 4 6 3 Parameters in DNA Studio Nozzle type The nozzle type includes the size of the nozzle needle inner diameter Changing the nozzle type will result in th...

Page 92: ...to set between 0 200 ms Post flow delay The amount of bioink that stop extrusion before the end of the filament or before the last coordinate of the filament Increase post flow delay if extrusion need...

Page 93: ...elay can extend the print time which can be stressful for the cells Figure 65 A B C Adjusting the post flow delay A short B enough C long delay P2 There is a gap at the start of the filament Increase...

Page 94: ...BIO X6 User Manual v 1 0 94 05 Gcode Editor...

Page 95: ...from scratch is often time consuming With this tool you can import and edit existing Gcodes or create a new Gcode based on existing Gcodes The Gcode Editor also allows you to check the Gcode syntax to...

Page 96: ...1 0 96 5 1 Launching the Gcode Editor Tap the Gcode Editor button on the home screen A new Gpad window opens Alternatively go to the Windows search bar type Gpad and Enter Figure 68 Gcode Editor tool...

Page 97: ...ve the updates made in the existing Gcode file 2 Save as Tap to save the new existing file under a new file name 3 Open file Tap to open an existing Gcode file 4 Create new Tap to create a new Gcode f...

Page 98: ...0 Actions bar in the Gcode Editor 6 Redo Tap to redo the changes or updates you have made 7 Undo Tap to undo the changes or updates you have made 8 Save icon Tap to save the changes Alternative to the...

Page 99: ...isualize the coordinate system the travel moves and the chosen surface Figure 71 View options Show coordinate system Enable this toggle button to show the coordinate systems on the screen for the X Y...

Page 100: ...4 Find and replace Use the Find and replace feature to quickly modify your Gcode Here you can see the number of Gcodes entries with the same description and replace one several or all entries to see h...

Page 101: ...of your code 1 Tap the Right end arrow to manually move forward to the next line of code 2 Tap the Left end arrow to manually move to previous line of code and undo the travel move 3 A line by line pr...

Page 102: ...ll moves toggle button is enabled 8 Stop Preview Tap to stop the preview of travel move 9 Toolhead bar Display the tool heads to be used in the bioprinting process Each tool is given a color for ident...

Page 103: ...parameters to provide if any and a description of the purpose of the selected Gcode Figure 76 Gcode Syntax description When using any G M or T commands that are not allowed or that are not part of the...

Page 104: ...BIO X6 User Manual v 1 0 104 Figure 77 Error message Command not supported...

Page 105: ...BIO X6 User Manual v 1 0 105 06 Draw Print...

Page 106: ...properties and infill without any CAD or Gcode input Free draw structures with lines and create squares circles and polygons shapes Each line or shape is shown in the same color as the color code for...

Page 107: ...ss and indicates in blue the step you are currently in 2 Print surface Select the surface you wish to draw and print on Petri dish well plate or glass slide 3 Preview The printing surface currently se...

Page 108: ...r thickness Enable this toggle button to apply consistent layer thickness to all layers 2 Layer thickness Tap to specify the thickness of each layer in mm The default value for layer thickness is 0 41...

Page 109: ...e screenshot below Figure 81 Consistent layer thickness disabled 5 Duplicate layer Tap to create a duplicate layer This will add a layer on top of previous layer When the Consistent layer thickness to...

Page 110: ...e which is already drawn on the surface 2 Circle Tap and drag the pointer on the surface to draw a circle 3 Square Tap and drag the pointer on the surface to draw a square 4 Polygons Tap chose the req...

Page 111: ...urface Snap to grid Enable the toggle button to place your structure in the grid This helps you improve accuracy when free drawing a shape Note Enable the button before you draw to make your structure...

Page 112: ...u want to use to print the shape 2 Width Select the required shape with the selector tool Tap to change the width of the shape Alternatively Select the shape hold the mouse pointer on the corner squar...

Page 113: ...d select Paste to insert Duplicate Select Duplicate to make a quick copy of the selected shape The copy is automatically inserted with a small offset to the original shape 7 Trash bin Tap to delete th...

Page 114: ...applied to all selected shapes When you select multiple shapes with different density the density value box turns into a dropdown and displays Mixed The dropdown shows a list of all densities of the...

Page 115: ...different dimensions 2 3D The 3D view is the standard view for printing 3D models see Bioprinting from a 3D model Use the slider and isometric views to show all the layers in 3D 3 Draw Draw is an orth...

Page 116: ...t right up and down These options are only available in the 3D view mode 3 Zoom in and Zoom out Tap these icons zoom in or out These options are available in both 3D and Draw view mode 6 2 6 Gcode gen...

Page 117: ...BIO X6 User Manual v 1 0 117 07 Shape Generator...

Page 118: ...ble value When any of the dimension chosen is less than 1 19mm size an information of which gauge should be used will be shown In the example below are the settings available for the predefined shape...

Page 119: ...of the shape 7 Confirm Tap to confirm the chosen shape and dimensions 8 Cancel Tap to discard the settings made 9 Diameter Specify the diameter for the cylinder and hemisphere shapes see screenshots...

Page 120: ...and moving it 3 Tap the trash bin to delete the selected file Any modifications can be reset or previewed in the center of any print surface 4 Previous Tap to go back to the previous step in the biopr...

Page 121: ...zoom in and zoom out This option helps you visualize with more detail the models to print and check for issues on the model or with the slice 8 Preview The model and its organization on the printing s...

Page 122: ...BIO X6 User Manual v 1 0 122 08 Print report...

Page 123: ...ust be connected to DNA Studio during the entire print to successfully autogenerate and store a print report Disconnection during the printing process will require manual print report generation from...

Page 124: ...Opening and adding comments to print report Figure 95 Print complete dialog In the Print complete dialog Tap Add print comments to add any comments to your print report Tap Open print report to view t...

Page 125: ...nual v 1 0 125 8 3 Information included in the print report Below you find some examples of what information is included in the print report and how it is presented lists tables and charts Figure 96 P...

Page 126: ...BIO X6 User Manual v 1 0 126 Figure 97 Protocol setup continued...

Page 127: ...BIO X6 User Manual v 1 0 127 Figure 98 Example of data included in print report printhead pressure and print speed...

Page 128: ...BIO X6 User Manual v 1 0 128 Figure 99 Example of data included in print report printbed temperature Figure 100 Example of data included in print report chamber temperature...

Page 129: ...er Manual v 1 0 129 8 4 Opening previous print reports Tap in the home screen and tap Print Reports to open stored PDF or HTML print reports Figure 101 Print Reports option in the left menu on the hom...

Page 130: ...BIO X6 User Manual v 1 0 130 09 Coaxial printing...

Page 131: ...coaxial feature is only available for BIO X6 9 1 Coaxial set up The coaxial printing set up is made in the Printheads step in the Model protocol On the home screen tap Model choose surface to print on...

Page 132: ...included in the coaxial kit to adjust it CELLINK offers different coaxial kits that contain everything you need to get started with coaxial bioprinting including one 0 5 inch coaxial nozzle suitable f...

Page 133: ...d pair third and fourth printhead Third pair fifth and sixth printhead Coaxial printing can be done with pneumatic printheads and temperature controlled printheads in any combination see screenshot be...

Page 134: ...BIO X6 User Manual v 1 0 134 10 Maintenance...

Page 135: ...er according to the instructions in Section 10 3 of this manual Every day before and after use Sensors and stop statuses Check the sensors by triggering each sensor manually and checking the status in...

Page 136: ...nter with the latest software version 1 Log into your MyCELLINK account at my cellink com If you do not have an account follow the steps online to register Note You need to provide the serial number o...

Page 137: ...BIO X6 User Manual v 1 0 137 9 Reboot the BIO X6 after the software update is complete If you run into any issues please contact support cellink com...

Page 138: ...the voltage input on the outlet to ensure it matches the required voltage onthe back of the BIO X6 The printbox is not moving or freezes during print Bioprinter is not homed Home the bioprinter in th...

Page 139: ...nd andconnected Unable to connect to the printer s Wi Fi Ensure the Wi Fi configuration is correct The hot spot name is the serial number of the BIO X6 Password cellinkbiox6 Wi Fi connection fails Be...

Page 140: ...l air supply The air supply is interrupted Check the connection and make sure it is connected correctly without leakage If compressor is being used ensure the compressor works as intended and supplies...

Page 141: ...move Hardware on your computer before removing the USB flash drive USB file doesnot appear USB file error Try a different USB Format the USB flash drive Redownload files to the USB flash drive UV ligh...

Page 142: ...0 142 be is too tilted more than 2 degrees Check the correct position and fixing of the surface petri dish well plates with the printbed Add the Petri dish holder kit to avoid any movement on the sur...

Page 143: ...ury Task Action Regularity Check Dates Clean door in out Clean with a smooth cloth Do not use harsh solvents The glass is sensitive to acetone and other chemicals Every week Clean top plate Clean care...

Page 144: ...irt and dust Every 3 months Clean Pneumatic Printhead Clean the printhead with a smooth cloth to remove any dust and debris When not being used store the printhead inside a sealed plastic bag in a coo...

Page 145: ...tion or deterioration of the instrument Every time it is used Clean mirrors metal reflectors The ultrasound sensors are very sensitive elements that help the BIO X6 self calibration process Any type o...

Page 146: ...BIO X6 User Manual v 1 0 146 11 Terms and conditions...

Page 147: ...ase CELLINK s General Terms shall prevail Email shall be considered sufficient wherever written is used in these General Terms 11 1 1 Orders and supplies etc The Buyer shall order the Products in writ...

Page 148: ...to Buyer from CELLINK under this Agreement or any other agreement In the event that the Buyer at any time should fail to make payment in full on the due date CELLINK shall be entitled to claim intere...

Page 149: ...y such claims Subject to the limitations stated above CELLINK is liable for claims made by a consumer in accordance with mandatory law for personal injury or damage to property caused by the Product I...

Page 150: ...LLINK in accordance with the provisions of Section 5 3 CELLINK is only liable for defects that appear under the intended and proper use of the Products included printing using CELLINK s bioink only Th...

Page 151: ...defects save as stipulated above This applies to any loss the defect may cause such as loss of production loss of profit and other consequential economic loss The limitation of CELLINK s liability un...

Page 152: ...intellectual property rights The Buyer shall not remove or change any trademark trade name sign or other mark on any Products or its packing or make any alterations in the construction or design of an...

Page 153: ...ilar importance shall be considered as Discharging Circumstances If a Party wishes to invoke a Discharging Circumstance it shall give immediate notice to the other Party of the commencement and the ce...

Page 154: ...rns Returns not pre authorized will not be eligible for repair replacement or refund Do not send it back to CELLINK unless you have contacted us first and we have authorized you to send it 11 1 15 Mis...

Page 155: ...e modifications have become effective shall be deemed licensee s conclusive acceptance of the modified terms CELLINK holds all right title and interest in and to the CELLINK 3D Bioprinter Software Lic...

Page 156: ...es updates or revisions to the Licensed Software however such updates may be introduced by CELLINK from time to time The Licensed Software may communicate with CELLINK or its third party provider serv...

Page 157: ...l over and is not responsible for the terms and conditions or privacy policies of any third party websites and makes no representations whatsoever about any other website that may be accessed by Licen...

Page 158: ...emove Licensee s information or content at any time from CELLINK s or its service provider s hosting platforms in CELLINK s sole discretion and without prior notice to Licensee Licensee agrees and may...

Page 159: ...veloping or printing any designs such as designs for use with a CELLINK 3D Bioprinter Licensee agrees to abide by all applicable laws and regulations and not to violate any third party right including...

Page 160: ...D Bioprinter Licensee agrees not to settle any such matter without the prior written consent of CELLINK For any matter which Licensee is required to indemnify CELLINK CELLINK at its sole option may a...

Page 161: ...r License or other terms of service are void and have no effect unless agreed to in a writing signed by CELLINK Any other modification to this User License must be made by written instrument duly exec...

Page 162: ...r License shall not be construed as a waiver of any provision or the relinquishment of any rights QUESTIONS AND INFORMATION Questions can be addressed and additional information can be obtained about...

Page 163: ...work out the problem on an individual basis as each problem must be addressed this way In the rare case of a specifically diagnosed and proven DOA dead on arrival part detailed description and docume...

Page 164: ...and warranty cover critical components only For example CELLINK will not replace a bioprinter because of a perceived blemish in the paintjob The critical components replacement parts do not carry a w...

Page 165: ...Deductions will be made for any wear perceived to the bioprinter including scratches The 14 day period after receiving the bioprinter will be concretely determined in calculation with the FedEx tracki...

Page 166: ...BIO X6 User Manual v 1 0 166 12 Printhead manuals...

Page 167: ...rusion based printhead It is equipped with heating up to 60 C It employs compressed air to extrude bioinks The Standard Pneumatic Printhead is designed to fit 3 mL cartridges only The current temperat...

Page 168: ...emperature red is hot yellow is normal and blue is cool 3 Opening for cartridge overview View the volume of bioink left in the cartridge 4 Cartridge fastening screw Securely fastens the cartridge to t...

Page 169: ...t the cartridge in the printhead Push it down until the cartridge wings touch the top of the printhead 4 Turn the cartridge fastening screw to secure the cartridge in the printhead Note The Standard P...

Page 170: ...ture up to 17 C below the chamber temperature It employs compressed air to extrude bioinks The Temperature controlled Printhead is designed to fit 3 mL cartridges only and is recommended for bioprinti...

Page 171: ...ion DO NOT put fingers or other objects into the fan while the printhead is in operation Doing so risks injury and damage to the printhead 12 2 2 Printhead overview Figure 110 Overview of the Temperat...

Page 172: ...ture of up to 250 C It employs compressed air to extrude thermoplastics Since most of these materials have a high viscosity when melted it is recommended to print them with the Thermoplastic Printhead...

Page 173: ...iew Figure 112 Overview of the Thermoplastic Printhead 1 Printhead body 2 Stainless steel cartridge 3 Top cap 4 Insulated locking ring 5 Stainless steel plunger 6 Plunger removal tool 7 Wrench for tig...

Page 174: ...thead 1 Place the stainless steel plunger inside the metal cartridge and attach the top cap 2 Insert the assembled cartridge into the printhead Twist until the cartridge is screwed in place and use th...

Page 175: ...BIO X6 User Manual v 1 0 175...

Page 176: ...BIO X6 User Manual v 1 0 176 Create the future of medicine...

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