Carolina 3M Petrifilm ColiformCount Plates Instruction Manual Download | Manualshive

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82-4030

3M™ Petrifilm™Coliform

Count Plates

Instruction Manual

CAROLINA

®

Teamed with Teachers

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Summary of Contents for 3M Petrifilm ColiformCount Plates

Page 1: ...82 4030 3M Petrifilm Coliform Count Plates Instruction Manual CAROLINA Teamed with Teachers...

Page 2: ...for counting the viable organisms present See Method A Petrifilm plates can be hydrated first with water or buffer then inoculated by swabbing streaking or touching to surfaces See Method B Antibioti...

Page 3: ...g Petrifilm plates Disinfect the work area by wiping thoroughly with alcohol or other disinfectant before and after use Used Petrifilm plates may contain viable organisms Do not open the plates unnece...

Page 4: ...cular to the Petrifilm plate place 1 mL of sample onto the center of the bottom film fig 3 If necessary samples can be diluted with distilled water liquid culture medium or buffers with pH between 6 6...

Page 5: ...gure 4 5 With the flat side down not the side with the circular ridge place the spreader on the top film over the inoculum fig 5 Figure 5 6 Gently apply pressure on the spreader to distribute the inoc...

Page 6: ...tion time and temperature will vary according to the application and equipment available 9 Colonies on Petrifilm plates can be counted on a standard colony counter or other light source fig 8 Bacteria...

Page 7: ...diluents that contain citrate or sodium thiosulfate because they can inhibit growth on the Petrifilm plates These substances are not found in common microbiological media such as Luria broth or nutri...

Page 8: ...media such as Luria broth or nutrient broth Interpretation The U S Food and Drug Administration Bacteriological Analytical Manual defines coliforms as gram negative rods that produce acid and gas from...

Page 9: ...9 Sample Plate Figure 12 Figure 11 Gas bubbles produced by colonies Gas bubble not associated with a colony...

Page 10: ...BiologicalSupplyCompany 2700 York Road Burlington North Carolina 27215 CB162760002 To order call 1 800 334 5551 US and Canada 336 584 0381 International For technical help call 1 800 227 1150 www caro...

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