Bruker ultraflex Series Maintenance Manual Download Page 1

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ultraflex Series

Cleaning Manual

Revision 2 (January 2013)

Summary of Contents for ultraflex Series

Page 1: ...ultraflex Series Cleaning Manual Revision 2 January 2013...

Page 2: ...o change without notice Bruker Daltonik GmbH makes no warranty of any kind with regard to this material including but not limited to the implied warranties of merchantability and fitness for a particu...

Page 3: ...r use in diagnostic procedures Hyperlink Disclaimer Bruker Daltonik GmbH makes no express warranty neither written nor oral and is neither responsible nor liable for data or content from the linked In...

Page 4: ...g label A triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument are as follows Toxic material Electric shock RF...

Page 5: ...ces of not heeding the warning or caution Read all warnings and cautions carefully and observe them at all times Their format is WARNING A Warning message appears in the manual when failure to observe...

Page 6: ...rce Heater 13 2 Introduction 15 2 1 LC MALDI 15 2 2 Imaging 15 2 3 Tools and Materials Required 17 3 Switching Off and Opening the Instrument 19 3 1 Preparation 19 3 2 Switching Off the Instrument 19...

Page 7: ...GmbH Table of Contents 5 Closing and Switching On the Instrument 39 5 1 Check HV Connections 39 5 2 Closing the Source 40 5 3 Switching On the Instrument 42 ultraflex Series Cleaning Manual Revision...

Page 8: ...Bruker Daltonik GmbH Document History Revision Date Changes Approval 1 03 01 2011 First Edition S Hasemann 2 11 01 2013 Add laser safety information S Hasemann Page 8 of 44 ultraflex Series Cleaning M...

Page 9: ...he responsible person must ensure that the Maintenance Instructions to be used by the person entrusted with equipment maintenance are available in a complete and legible form before and during the mai...

Page 10: ...nstrument is switched on WARNING DANGER OF CHEMICAL BURNS Depending on the use to which the instrument has been put the cleaning process may expose the user to harmful chemicals Wear appropriate eye p...

Page 11: ...slip hazard Ensure that all spills are cleaned up immediately Note Always allow sufficient time for maintenance procedures As a guide 2 hours is the minimum that should be allowed Note Make sure that...

Page 12: ...trument For further information about the smartbeam II laser system refer to Holle A Haase A Kayser M H hndorf J Optimizing UV laser focus profiles for improved MALDI performance Journal of Mass Spect...

Page 13: ...Source heater warning label Note Because the instrument will be disconnected from the power supply there is no risk from laser light if the working steps and instructions given in this manual are foll...

Page 14: ...1 Safety Instructions Bruker Daltonik GmbH Page 14 of 44 ultraflex Series Cleaning Manual Revision 2...

Page 15: ...I Source heating interval l Cleaning threshold 5 million shots default as shown in Figure 2 1 Manual cleaning interval l Weekly Flushing l Monthly Clean the optical components using a swab see section...

Page 16: ...oduction Bruker Daltonik GmbH Figure 2 1 Cleaning Information dialog settings LC MALDI Figure 2 2 Cleaning Information dialog settings MALDI imaging Page 16 of 44 ultraflex Series Cleaning Manual Revi...

Page 17: ...er 8 201106 Safety glasses Safety glasses with side protection Tissues Free of any impregnation for exam ple Kimberly Clark KIMTECH SCIENCE Laboratory Tissues Bruker 8 321559 Swab for cleaning optics...

Page 18: ...2 Introduction Bruker Daltonik GmbH Page 18 of 44 ultraflex Series Cleaning Manual Revision 2...

Page 19: ...damaging your samples l Move the sample holder to position A24 to enable better access to the P1 cable contact pole l Close flexControl and flexService l The external nitrogen supply must be opened t...

Page 20: ...ey switch on the control panel at the front of the ultraflex o Turn the key one quarter turn counterclockwise to the horizontal position o The pumps the high voltage and the lasers are now switched of...

Page 21: ...rument cannot be switched on during maintenance o The system starts venting immediately after the instrument is switched off The sound of the decelerating turbo pumps are an indication that this is ha...

Page 22: ...7 Rear view of ultrafleXtreme l Activate the top cover lift buttons with the key switch on the panel at the rear of the instrument o Turn the key to the vertical position to activate the top cover lif...

Page 23: ...Bruker Daltonik GmbH 3 Switching Off and Opening the Instrument Figure 3 8 Rear panel and inset top cover lift buttons and enable key switch ultraflex Series Cleaning Manual Revision 2 Page 23 of 44...

Page 24: ...ting jack has an overtemperature safety switch If overused frequent up and down movement the thermostatic cut out will prevent it from working until it cools down l Switch off the instrument using the...

Page 25: ...the nitrogen supply to prevent wastage l Open the door of the source chamber using the 5 mm Allen key see Figure 3 11 o First loosen and remove the screws on the hinge side left side o Second loosen...

Page 26: ...connected right disconnected WARNING DANGER OF ELECTRIC SHOCK Before proceeding make sure that the mains connector is disconnected to avoid the risk of an electrical shock 3 4 Components Inside the S...

Page 27: ...Bruker Daltonik GmbH 3 Switching Off and Opening the Instrument Figure 3 14 The ion optics ultraflex Series Cleaning Manual Revision 2 Page 27 of 44...

Page 28: ...3 Switching Off and Opening the Instrument Bruker Daltonik GmbH Page 28 of 44 ultraflex Series Cleaning Manual Revision 2...

Page 29: ...se appropriate safety equipment Refer to local Biohazard regulations and consult the Safety Representative before starting work Note It is very important to wear appropriate personal protective equipm...

Page 30: ...mination causes a reduction in laser energy arriving at the target meaning that the laser energy must be increased using the software setting The laser mirror on the mirror plate is the component that...

Page 31: ...ing procedure Note Always soak cleaning materials in ethanol Do not clean parts with dry tissues swabs or brushes Figure 4 2 Flushing the upper surface of the laser lens Flush the upper surface of the...

Page 32: ...s ethanol using a tissue Roll the tissue into a tight cylinder so that pressure can be applied to the surface 4 1 3 Mirror Plate Figure 4 6 Flushing the ion optic mirrors using ethanol Flush the ion o...

Page 33: ...tics Flush mirrors and lens of the camera optics with ethanol using the wash bottle 4 1 5 Illumination Optics Figure 4 9 Mirrors and lens of the illumination optics Flush mirrors and lens of the illum...

Page 34: ...Example of a heavily contaminated P2 plate l The P2 plate is very sensitive do not touch the surface directly as this may bend the plate Use a brush Bruker Part No 228209 soaked in ethanol to clean th...

Page 35: ...the P2 plate from the front Figure 4 12 Flushing the P2 plate from the rear Figure 4 13 View through the observation window while flushing the rear side of the P2 plate and the ground plate from both...

Page 36: ...plate and behind the lens plate try to push the brush completely through Note Always soak cleaning materials in ethanol before starting cleaning Do not clean parts with dry tissues swabs or brushes Af...

Page 37: ...flow combined with source heating The source heating is unable to remove all contamination Frequent manual cleaning is an absolute necessity 4 3 Final Tasks Dry the parts inside the source using nitro...

Page 38: ...4 Cleaning the Source Bruker Daltonik GmbH Page 38 of 44 ultraflex Series Cleaning Manual Revision 2...

Page 39: ...ctions Before closing the source check that the Lens P2 and P1 shield cables are still in place see Figure 5 1 Figure 5 2 and Figure 5 3 Figure 5 1 Connection to the Lens plate Figure 5 2 Connection t...

Page 40: ...debris such as dust fluff hair and so on that could cause a sealing problem l Before closing the source door check the position of the O ring around the door Trapping the O ring when it is unseated ca...

Page 41: ...e 5 5 Close the door almost completely to be able to reconnect the P1 connector to the contact pole l Close the door completely and insert and tighten the source door screws o Tighten the screws on th...

Page 42: ...h is switched to the ON position Figure 5 8 Keep hands well clear of the closing top cover WARNING PINCH HAZARD The closing cover presents a pinch hazard Keep all body parts away from the descending t...

Page 43: ...5 10 It will take approximately 15 seconds for the indicators on the control panel to light up l The pumps and electronics should now start Figure 5 10 Control panel switched on key is vertical l Sta...

Page 44: ...er Daltonik GmbH Figure 5 12 The Vacuum Check Pressure window Note After the cleaning procedure it takes approximately 4 hours to restore the vacuum and for the system to be ready for use Page 44 of 4...

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