Branson Sonifier 250, User Manual, Page: 113 / 135

Application Information
A-12 100-214-239 Rev. A
Retinal outer segments:
ultrasonic treatment breaks particles down to almost molecular size.
Rhodopseudomonis palustris:
complete disruption in 4 minutes.
Rhodospirillum rubrum:
excellent disruption in a few seconds.
Rimosus:
monocellular elements from surface-grown colonies obtained in 1 minute.
Complete disruption in 5 minutes, 50% disruption in 2 minutes.
RNA:
rapid and thorough re-suspension of 9 TCA pellets during extractions.
Rocks:
excellent for disaggregation of sedimentary rock and for cleaning material rock sur-
faces between polishing stages.
S. faecalis:
excellent disruption in 1 minute.
S. fragilis:
5 minutes yielded excellent release of galactokinase, more than any other method.
Subcellular particles may be extracted or disrupted.
Saliva glands:
complete disruption.
Salmonella:
various culture media or phosphate buffered saline disintegrated between 40 and
50% in 10-20 minutes. Sonifying was one step in an improved assay for enzyme
thiogalactosize transacetylase.
Salmonella typhimurium and enteritidis:
bacteria were suspended in 1/300 volume of orig-
inal culture, sonified for 4 minutes and centrifuged for 20 minutes at 20,000 g. Extracts were
found to catalyze the synthesis of cytidine diphosphate 3, 6-dideozyhexoses.
Scholasticism mansion:
complete disruption.
Sedimentary rock:
completely disperses flocs with the release of all bound silt and clay
particles.
Sediments:
ultrasonic treatment disperses fine material permitting quick, neat separation of
sand from silt and clay fractions.
Serial number restoration:
used in crime laboratories to restore obliterated serial numbers.
Serratia marcescens:
complete breakdown of a concentrated solution in 1 minute.
Serum:
quickly homogenized.
Serum cholinesterase:
activated by ultrasonic treatment. Different cholinesterase isoenzymes
may be activated and inactivated selectively.
Shale:
excellent disaggregation of all fine-grained sedimentary rocks.
Shellfish:
by drilling a clean hole with the micro tip, various fluids or samples may be
withdrawn or injected from living shellfish without destroying the animals.
Shigella:
quick disruption.