Branson Sonifier 250, User Manual, Page: 111 / 135

Application Information
A-10 100-214-239 Rev. A
Micrococcus lactiliticus:
75 ml of a 20% suspension was disintegrated in 15 minutes and a
good yield of the enzyme Xanthine dehydrogenase extracted.
Mineral rock:
excellent for cleaning surfaces between polishing stages.
Mitochondria:
separate from cells without injury. Mitochondria themselves can be broken
with longer ultrasonic treatment. Inner membrane subunits also isolated.
Muscle tissue:
1 gm homogenized in 4 minutes; heart muscle in 6 minutes.
Mycobacteria:
a 20 ml growing media completely disrupts in 14 minutes. Clumps break
quickly. An immunological compound prepared.
Mycoplasma antibody:
a suspension of Campo-W cells treated for 5 minutes gave 12 lines
with the sera in a gel diffusion test. The extract was estimated to contain 12.75 mg protein per
ml by Blaret reaction.
Myeloma tumor cells:
complete disruption in 10 minutes, 30% disruption in 2 minutes.
Myleran:
made colloidal suspension and dissolved in approximately 1 minute.
N. crassa:
nuclease was isolated and purified from conidial extracts after 5 minutes
treatment.
Naegleri gruberi:
this free-living soil amoeba was treated ultrasonically to release subcellular
infectious material.
Neurospora:
40 ml, 4 minutes, produced more protein than freeze thawing for study of
enzymatic synthesis of cystathionine.
Nocardia ostenodes:
breaks clumps and disintegrates in less than 10 minutes.
Nucleoprotein:
extracted from tissue. May be degraded selectively.
Oil and water emulsions:
permanent, stable emulsions in a few seconds. Particle size reduced
to less than micron (each case slightly different). Oil in water/water in oil phases can be
obtained in same vessel.
Oyster shell:
small, clean hole can be drilled with micro tip in 3 minutes. No cracking is
produced.
Paracolon:
excellent disruption.
Parasites:
separated from red blood cells in a few seconds.
Pasteurella pestis:
complete disruption in 30 minutes using high power.
Penicillium:
complete disruption in 3 minutes.
Pesticides:
ultrasonic treatment resulted in a 16-fold improvement in the potency of the
antigen used with Microcrystalline Cellulose as a thin-layer absorbent for chromatographic
separation.