Branson Sonifier 250, User Manual, Page: 110 / 135

100-214-239 Rev. A A-9
Appendix A
Digital Sonifier
Hydrophilic vegetable gums:
disperses and solubilizes hydrophilic vegetable gums in water;
makes dispersions of added particulate matter.
Intracellular membrane:
disruption and particle size reduction obtained in 30-60 seconds.
Isoenzymes:
selectively activated with respect to time and intensity of treatment.
Kidney:
1 gm disintegrates in 3 minutes.
Kidney stones:
easily broken in seconds in vitro.
Klebsiella:
excellent disruption.
L. arabinosis:
complete disruption to free virus in 2 minutes without injury.
Lactobacillus:
0.5 gm in 15 ml completely disrupts in 11 minutes. Excellent release of
acetokinase.
Lenconostoc mesenteroides:
ultrasonic treatment for 15 minutes using high power for dis-
ruption.
Leukocyte lysozyme activity in myelocytic leukemia:
the cell suspension was ultrasonically
treated and samples assayed for lysozyme activity. The lysozyme concentration of the leuko-
cytes ug/10
6
cells was determined.
Linoleic acid:
made suspension in water in 30 seconds.
Liver tissue:
1 gm homogenizes in less than 1 minute.
Lung tissue:
1 gm homogenizes in 2 minutes.
Lymphacytis:
complete disruption in 15 seconds.
Lymphocyte nuclei:
complete disruption in 6 minutes.
Lymph gland:
direct injection lymphography with a modified radiopaque emulsion was
obtained by ultrasonic treatment in a functional procedure producing lymphatic structure
detail.
Lysossomes:
released enzymes quickly.
Malaria prolozoa:
fast, excellent disruption.
Maple bark spores:
complete disruption in 14 minutes.
Measles:
disruption of virus antigen clumps present in infected cells on low power.
Ultrasonic treatment increased antigen titer 4-8 fold.
Methanobacillus omelianskii:
1 gm cells wet wt/ml completely disintegrates in 2 minutes
for assaying methane.
Microbacterium lacticum:
ultrasonic treatment used for malate dehydrogenase extraction.
Micrococci:
a 13 ml solution completely disrupts in 15 minutes.