Branson Sonifier 250, User Manual, Page: 109 / 135

Application Information
A-8 100-214-239 Rev. A
Eugoena:
complete disruption in 12 minutes, 90% disruption in 8 minutes with pigment
released.
Fat extraction:
fat can be emulsified without injuring tissue with proper power selection. Lipid
layer can be stripped from spores and mycobacteria.
Fibrin:
complete suspension of 0.125 gm in 30 minutes.
Fish gill:
complete disruption of 20 mg in 30 seconds.
Fish tissue:
tissue homogenization for extractions and excellent particle size reduction in 8
minutes per 10 gm.
Fluorocarbons:
extended treatment time will break down particle size to well under 1 micron
and gives a fine homogenate.
Fossils:
low power will clean debris from delicate fossils without injury. Micro fossils such as
pollen can be separated from rocks to help identify the geological age of the strata. Removal
of rock matrix.
Gamma globulin:
the Sonifer Cell Disruptor was used to solubilize protein as one of the steps
in the biosynthesis of gamma globulin from rabbit spleen.
Gangliosides:
immunochemical and structure studies used ultrasonic treatment as one step.
Gastric mucosa:
placing scrapings into a test tube and test tube into new water-filled cup horn
caps permits these cells to be separated without breakage.
Graphite molybdenum disulfide:
an excellent dispersion of this lubricant was made in a
silicate binder.
Guanine:
produces colloidal suspension in 1 minute.
Gymnodinium:
solution completely disrupts in 6 minutes.
Haemophilus pertussis:
preparation of successful immunological complexes.
Heart muscle:
1 gm disintegrates in 6 minutes.
HeLa cells:
disruption to free virus in a few seconds without injury.
Hemophilus pertussis:
an immunological compound prepared.
Herpes virus:
may be quickly released without injury.
Histoplasma capsulatum:
ultrasonic treatment for 7 minutes completely ruptures cells
prepared by formalin fixation. Good enzyme activity is obtained.
Human serum proteins:
ultrasonic treatment causes a reproducible change in the electro-
phoretic behavior of normal human serum consisting of an increase in material migrating in
the x and b globulin zones with a reduction in the albumin and y globulin fractions.
Hydrocortisone:
smaller crystals were produced by ultrasonic treatment.