Branson Sonifier 250, User Manual, Page: 107 / 135

Application Information
A-6 100-214-239 Rev. A
B. stereothermophilis spores:
complete disruption in 2 minutes.
B. subtilis:
disruption of 5 gm wet wt, 15 ml buffer, in 5 minutes.
B. subtilis veg cells:
heavy suspension clears in 1 minute.
Bacillus stereothermophulus (thermophillic spore form)
: 98% disruption of 70 ml of
40% suspension in 15 minutes.
Bacillus brevis:
1:15 W/V in 3 minutes.
Bacteroides Symbiosis:
1-phosphorfructokinase (a soluble enzyme) has been isolated from
this anaerobe by ultrasonic treatment. A 25 ml suspension was sonified for 10 minutes and
centrifuged at 36,000 xg for 10 minutes.
Baker’s yeast (saccharomyces cerevisiae):
see Yeast.
Blastomyces dermatitidis:
95% disruption in 3 minutes.
Blood cells:
red and white cells can be disrupted in a few seconds.
Boll weevil tissue:
complete homogenization in a few seconds.
Bone:
compact bone can be sonified and processed for microscopic sections in minutes.
Other methods can require up to a week. Bone specimens treated in this way yielded large
numbers of intact cells with little distortion. Malignant criteria are easily recognized. Tumor
types studied were: osteosarcoma, chondrosarcoma, liposarcoma, chordoma,
metastic bronchogenic squamous and benign giant. Bone can be decalcified without injury to
the cells, processed for microscopic sections, and diagnosed in a short time. Other methods
require extensive treatment time.
Brain stem and adrenal gland:
ultrasonic treatment dispersed 10 mg samples in 10 ml fluid,
which is usually difficult without substantial loss of material. The suspension was analyzed for
nucleotides.
Brain tissue:
disintegrates instantly.
Brevi bacterium:
25 ml disrupts in 20 seconds.
Brevi bacterium acetylicum:
approximately 3 minutes to disrupt large samples and
measure TCA enzyme activity.
Brine shrimp:
complete disintegration in 1 minute.
Brucella abortes:
separates easily from leucocytes. At least 9 antigens extracted.
Bull sperm:
contractile protein is easier to extract from tails after sonifying.
C. butyricum, C. cylinrosporum, C. kluyveri:
vegetative cells easily disrupted.
C. pasteurianum:
3 minutes disruption for hydrogens reducing Ferredoxin with H2.