Branson Sonifier 250, User Manual, Page: 105 / 135

Application Information
A-4 100-214-239 Rev. A
Disrupting Tissues and Solids
You can effectively homogenize or disrupt many kinds of tissue and other solids. Energy radi-
ates only from the horn’s tip. The energy is most concentrated within 1/2 inch (12.7 mm) of
the face of the tip. When you treat tissue or solids in solution, the freely moving cells or parti-
cles pass the face of the tip many times during the process. When you treat a solid piece, how-
ever, the energy pattern from the tip of the horn has a tendency to repel the solid away from
the tip. The solid does not receive treatment, but simply spins or circulates around the con-
tainer.
You can effectively treat all but the most difficult materials by following these two steps:
1. Homogenize the tissues or solids by placing them in a high-speed blender with the
solution.
2. Insert the horn in the liquid sample for complete disruption.
If you must disrupt solid pieces, especially those that are extremely resistant to breakage, with-
out homogenizing them, place the horn directly over the tissue or right against it.
Using Glass Powders with Solution
To disrupt difficult cells and tissues, adding glass powders (5 microns to 0.5 mm) will materially
decrease treatment times, especially when used in conjunction with the standard, high-intensity
microtip. A ratio of 1 part glass powder to 2 parts liquid is recommended.