Biotek Synergy HTX, Operator'S Manual

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Chapter 3: Getting Started
2.
Select the desired Dispenser number (1 or 2) and click
Initialize
. The
syringe drive will move to its home position and its sensors will be verified.
Upon successful completion, the Initialized field should show “Yes”.
Prime Utility
Before running an experiment with a Dispense step, the dispense module and its
associated tubing must be primed with the fluid to be used. Gen5 provides a
special utility for this task. To prime the dispense module:
1.
Fill the supply bottle with a sufficient volume of the fluid to be used for the
prime and the assay. Insert the appropriate inlet tube into the bottle.
2. Important!
Place the priming plate on the carrier.
3.
In Gen5, select
System > Instrument Control > Synergy HTX
and
click the
Prime
tab.
4.
Select the Dispenser number (1 or 2) associated with the supply bottle.
5.
Enter the Volume to be used for the prime, from 5 to 5000 µL.
The minimum recommended prime volume is 1100 µL.
6.
Select a prime Rate, in µL/second.
7.
Click
Prime
to start the process.
8.
When the process is complete, carefully remove the priming plate from the
carrier and empty its contents. If the priming plate is empty, the prime
volume was too low.
Purge Utility
Gen5 provides a special utility to purge fluid from the dispense tubing and syringe
by pumping the fluid in reverse, back into the supply bottle. To purge the dispense
module:
1.
In Gen5, select
System > Instrument Control > Synergy HTX
and
click the
Prime
tab.
2.
Select the Dispenser number (1 or 2) associated with the supply bottle.
3.
Enter the desired purge Volume in µL.
4.
Select a prime Rate in µL/second.
5.
Click
Purge
to start the process.
BioTek Instruments, Inc.