Biotek Synergy H1, Operator'S Manual

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Overview
A general Preventive Maintenance (PM) regimen for the Synergy H1 includes periodically
cleaning all exposed surfaces and inspecting/cleaning the excitation and emission filters
and mirrors (if equipped). For models with the external dispense module, additional tasks
include flushing/purging the fluid path and cleaning the tip prime trough, priming plate,
supply bottles, internal dispense tubing, and injector heads.
Daily Cleaning for the Dispense Module
To ensure accurate performance and a long life for the dispense module and injectors,
flush and purge the fluid lines with deionized (DI) water every day or after completing an
assay run, whichever is more frequent. Some reagents may crystallize or harden and then
clog the fluid passageways. Take special care when using molecules that are active at very
low concentrations (e.g., enzymes, inhibitors). Remove any residual reagent in the
dispense lines using a suitable cleaning solution (review the reagent’s package insert for
specific recommendations).
Flushing the tubing at the end of each day, letting the DI water soak overnight, and then
purging the lines at the beginning of each day ensures optimal performance of the dispense
system. BioTek recommends performing a visual inspection of the dispense accuracy
before running an assay protocol that includes dispense steps.
BioTek also recommends flushing the module with DI water before conducting the
decontamination procedure described in the
As-Needed Maintenance chapter.
Models with injectors : Accumulated algae, fungi, or mold may require
decontamination. See the As-Needed Maintenance chapter for
instructions.
54 | Chapter 5: Preventive Maintenance
BioTek Instruments, Inc.