Biotek Synergy H1, Operator'S Manual

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96-well and 384-well plate, sweep read speed
0–1 OD: ±1% ±0.010 OD
Repeatability
Using certified neutral density glass
Measured by one standard deviation (8 measurements/data point)
96-well and 384-well plate, normal read speed
0–2 OD: ±1% ±0.005 OD, delay after plate movement=100 ms
2–2.5 OD: ±3% ±0.005 OD, delay after plate movement=100 ms
96-well and 384-well plate, sweep read speed
0–1 OD: ±2% ±0.010 OD
Fluorescence Specifications
The Synergy H1 measures fluorescence from the top and bottom with monochromators,
and from the top with filters. The following sets of requirements apply to 96-well plates.
Monochromator-Based Fluorescence
Excitation Range 250–700 nm (with low-noise PMT)
250–900 nm (with red-shifted PMT)
Emission Range 250–700 nm (with low-noise PMT)
300–700 nm for emission scans (up to 900 nm with red-shifted PMT)
Bandpass ≤18 nm (Excitation and Emission)
Performance
Sodium Fluorescein in phosphate buffered saline (PBS)
DL ≤20 pM, top or bottom read
Excitation 485nm, Emission 528nm
Methylumbelliferone (MUB) in carbonate-bicarbonate buffer (CBB)
DL ≤0.16 ng/mL, top read
Excitation 360 nm, Emission 460 nm
Propidium Iodide (PI) in PBS
DL ≤62.5 ng/mL, bottom read
Excitation 485 nm, Emission 645 nm
142 | Appendix A: Specifications
BioTek Instruments, Inc.