Biotek Synergy H1, Operator'S Manual

Page: 139 / 190

Depending on the manufacture date of the test plate, the glass type may be
Erbium, Holmium, or Didymium. Contact BioTek TAC if you are not sure
which glass type is used in your plate.
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If the C6 filter is Erbium or Holmium glass, the certificate contains two Spectral
Bandpass tables.
Synergy H1 has a bandpass wider than 5 nm for wavelengths above 285
nm and smaller than 4 nm for 230–285 nm; we recommend you use the
expected peak values in the
5.0 nm table.
Erbium: Any peak value in the table can be used.
Holmium: For best results use the expected peak values closest to 242, 279,
362, 417, and 538 nm.
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If the C6 filter is Didymium glass, the certificate provides a single peak
wavelength value. Enter this value into Gen5 and set the Test Range – and +
values so the range displayed in parenthesis is "(580 to 590)".
8. Review all of the values that you entered. When finished, click OK to save the
information.
Test Procedure
1. In Gen5, select System > Diagnostics > Test Plates > Run . If prompted, select
the desired Test Plate and click OK .
2. When the Absorbance Test Plate Options dialog appears, enter any required
information.
3. If applicable, check the Perform Peak Wavelength Test box .
4. Highlight the wavelength(s) to be included in this test. Select only those wavelengths
most appropriate for your use of the reader.
5. (Optional) Enter a comment.
6. Click Start Test .
7. Place the Absorbance Test Plate on the microplate carrier, with A1 in the proper
location.
8. Click OK to run the test.
9. When the test completes, the results report will appear. Scroll down through the
report; every result should show "PASS".
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Troubleshooting tips are provided on page 76 .
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Test descriptions are provided on page 75 .
Synergy H1 Operator's Manual
Absorbance Plate Tests | 113