Biotech Sequence Precision Series, Instruction Manual, Page: 19 / 37

Sequence BioTech,
LLC
16
DISPENSING LIqUID FROM THE PIPETTE TIPS
•
Select the DISPENSE button on Control Panel . rotate the
Control Wheel to the desired dispensing volume. Press ENTER
on the Control Panel to begin dispensing. The display screen
will display:
“Dispense Volume” —the amount to be dispensed from each tip
“Current Volume” —the volume remaining in each tip
•
once all liquid has been dispensed from the pipette tips and
“Current Volume”
reaches zero,
“Raise Tips”
will appear on the
display screen.
•
Using the Levered Handle , raise the pipettor Head Assembly
upward to the highest position. Press ENTER once again to
return the instrument to the “ready position.” “Ready” appears
on the display screen.
MIXING LIqUIDS
•
ensure pipette tips are empty. Fully submerse tips into liquid
by lowering the pipettor
Head Assembly until the tips are deep
within the wells of the microplate (or other vessel). Using
MENU
on the Control Panel , select
“Mix”
and press
ENTER
. rotate the
Control Wheel to the desired mixing volume (approximately
half the volume in the wells) and press
ENTER
. Next, rotate the
Control Wheel to the desired number of mixing cycles (0-25
cycles).
The display screen will display:
“uL” —the mixing volume or the amount of liquid to be mixed
by each tip
“Cycles” —the number of desired mixing cycles
•
Press ENTER to begin the mixing cycles. While the pipettor
is mixing, the display screen will indicate the action until all
mixing cycles have completed.
•
once mixing has finished, press
ENTER
to return the instrument
to the “ready position.” “ready” appears on the display screen.
•
repeat aspiration, dispensing, and mixing steps as necessary.
EJECTING PIPETTE TIPS
•
With the pipettor raised to the highest position, slide the pipettor
Head Assembly into the notched guide in position one above
the empty tip rack. Lower the tips so they are positioned half
way into the holes of the rack. Press
EJECT on the Control Panel .
•
once the pipette tips have been ejected, lift the pipettor Head
Assembly to the highest position.
This ends the basic pipetting operation. To adjust the aspiration and
dispensing speeds on the Precision Series Pipettor,
see ADJUSTING
ASPIRATION AND DISPENSING SPEEDS .