Biotage Isolera Four, User Manual

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411829-L, Operation April 2012 Page 4-2
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4.3 Control the UV Lamp
The internal detector uses an ultraviolet (UV) lamp and, if using the UV-VIS detector, a tungsten lamp in
its detection process. The UV lamp must be warmed up in order for the signal to be accurate and stable.
NOTE
The UV lamp should be turned on 7.5 minutes before starting a purification
so the UV lamp can reach operating temperature.
The lamp(s) is/are automatically turned on when:
• The Isolera system is switched on.
• The user enters the Chemistry mode (i.e. press the Chemistry button in the main menu).
• The user starts a run (i.e. press the button in the
Run Parameters dialog). However, before
starting the run, the software waits (approximately 7.5 minutes) for the UV lamp to warm up.
You can also turn on the detector lamp(s) manually by pressing Turn Lamp On in the right-hand
panel. By doing this ahead of time, you can avoid the delay that is required if the software turns the
lamp(s) on at the start of a run.
To prolong lamp life, the lamp(s) is/are turned off automatically if the system is idle (no purification
is started and no user interaction is detected) for approximately two hours.
Bypass the Automatic UV Lamp Warm-up Period
You can bypass the automatic UV lamp warm-up period by pressing Skip Warm Up in the right-
hand panel. However, if the UV lamp has not reached operating temperature, purification results
may not be optimal.
4.4 Create, Open, and Edit Methods
4.4.1 Create or Open a Method
NOTE
If you want to use the method wizard, see page 4-13. The wizard will
guide you step-by-step through the setup of a purification with a gradient
including up to three steps using two solvents.
To scroll a list down or up, press or .
1. To create a new method:
a. Select the Method tab in the right-hand panel and press New... .
b. If the system is configured to use default methods (see “Set Default Methods” on page 2-4),
the New Method dialog opens. Select the default method that you want to base your method
on and press OK . (If there is only one default method, it will be opened when you press New .)
2. To open and edit an existing method:
a. Select the Method tab in the right-hand panel and press Open... .
b. If the method is stored in the system’s database, select the owner of the method in the
Open Method dialog. All methods of the selected user are listed. To select one of the
preconfigured Biotage methods, select the user “Biotage Methods”.
c. If the method is stored on a USB memory device, connect the device to the USB port
located underneath the touch screen and press in the Open Method dialog.
d. If the method is stored in the share folder on your network, press in the Open Method
dialog.
e. Select the method and press OK .