bioMerieux DENSIMAT, User Manual, Page: 35 / 50

User's Manual DENSIMAT 7-1
7 P r o c e d u r e
CAUTION! DENSIMAT is always OFF. It automatically comes ON when you insert an
ampoule and automatically comes OFF when you remove it.
•
Prepare a homogeneous bacterial suspension in the ampoule recommended
in the procedure for the reagent used.
•
Wipe the ampoule clean.
•
Place the ampoule in the well. DENSIMAT comes ON automatically.
•
Turn the ampoule until you obtain the minimum stable reading on the scale.
Only the minimum stable value obtained is of significance.
Note:
If you are using DENSIMAT with the AC power adapter, you should connect the
instrument to the power supply before starting these operations.
In order to save the batteries:
−
It is recommended to first homogenize the bacterial suspension and then place the
ampoule in the
DENSIMAT to perform the reading.
−
DENSIMAT goes into standby mode after 30 seconds if there is no change in the O.D.
of the suspension. This mode is indicated by “McF” on the display.
A d j u s t i n g t h e i n o c u l u m
Most standardized methodologies recommend the use of bacterial suspension
with a density equal to a McFarland value.
Therefore, it is often necessary to adjust the bacterial suspension to obtain the
recommended standard. The ampoule should remain in place while this
adjustment is being made.
−
If the density is higher than the standard required, the bacterial suspension
should be diluted by adding a certain volume of medium.
−
If the density is lower than the standard required, more bacteria should be
added.
Note:
In order to save the batteries, it is recommended to remove the ampoule from the
DENSIMAT to adjust the inoculum.