GeneExplorer Thermal Cyeler Operation Manual
Important Notes
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Chapter One Introduction
The chapter covers introduction to PCR technology and examples of its applications, introduction to
PCR thermal cyeler and features of GeneExplorer thermal cyeler.
1
Introduction to PCR technology
Polymerase Chain Reaction (PCR in short) is a nucleic acid amplification technology of in-vitro
simulation of natural DNA reproduction process. With two DNA chains to be amplified as a template and
mediated by a pair of artificially synthetic ODN primers, and through DNA polymerase of high
temperature resistance, it can rapidly and specifically amplify specific DNA segment. Due to its simplicity,
rapidity, peculiarity and sensitivity, ever since invention of PCR by Mullis in 1985 and discovery of DNA
polymerase of high temperature resistance by Erlich in 1988, over a few years, the technology has been
widely applied in various fields of bioscience. Especially in cytology, virology, oncology, genetic
epidemiology, forensic pathology and flora and fauna immunology, it has accomplished outstanding
achievements and become a milestone of contemporary molecular biology development.
2
Examples of PCR’s applications
Application of thermal cyeler: The product is applicable to polymerase chain reaction in fields
including immunology, human genome engineering, forensic pathology, oncology, organization and
population biology, paleontology, zoology and botany.
3
Features of GeneExplorer thermal cyeler
GeneExplorer thermal cyeler is a PCR of multiple functions and applications. It features as below:
Providing up to eight replaceable modules based on requirements of various PCR experiments;
Adopting advanced American ITI thermoelectric refrigeration technology and thermoelectric
refrigeration element manufactured under Japanese quality management mode which presents stable
and reliable performance;
Maximum ramp rate up to 4
C/s, and can be adjusted based on experiment requirements;
Multiple temperature control modes and higher reagent compatibility;
Host machine of GeneExplorer thermal cyeler can automatically recognize module and run
corresponding program;
Colorful large-screen display, humanized operation interface, simple and clear operation;
Power-off protection function, and after power supply recovery, it can continue to complete the
program unfinished;
Hot lid can adjust height and pressure based on actual using condition to adapt to different reaction
Chapter One Introduction
