Bio-Tek ELx800, Operator'S Manual

Page: 147 / 214

Liquid Testing
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119
Liquid Test 1
A 96-well, flat-bottom microplate is required for this
test (Corning Costar
 #3590 is recommended). Use a
new microplate; any fingerprints or scratches may
cause variations in readings.
IMPORTANT:
Before running the liquid tests, ensure
that the reader is not running in Rapid Mode. To
check this, select
UTIL READ
and then cycle
through the prompts until
READ IN RAPID MODE?
is displayed. Choose
NO
for an accurate result.
1.
Using freshly prepared stock solution A or B (the
concentrated
solution), prepare a 1:2 dilution using DI water (one part stock, one
part DI water; the resulting solution is a 1:2 dilution).
2. Pipette 200
µ
l of the concentrated solution into Column 1 of the
plate.
3. Pipette 200 µl of the diluted solution into Column 2 of the plate.
4.
Read the microplate five times at 405 nm using the Normal Read
Mode (
Chapter 3 ), single wavelength, no blanking.
5. Print the five sets of raw data or export it to an Excel spreadsheet
using KCjunior™ or KC4™.
If you are exporting the data to your own Excel spreadsheet,
perform the calculations described below and keep the
spreadsheet for future tests.
Calculations:
6. Calculate the mean and standard deviation for each well location in
columns 1 and 2 for the five plates read. Only one data set needs to
be analyzed for each concentration. The well that shows the most
variation for each concentration is selected for data reduction.
7. For each mean below 2.000 OD, calculate the allowed deviation
using the repeatability specification for a 96-well format of
± 0.5% ± 0.005 OD.
ELx800 Operator's Manual