Bio-Tek ELx800, Operator'S Manual

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Chapter 4: Performance Verification and IQ/OQ/PQ Procedures
Absorbance Plate Test
Note: Before running the Absorbance Plate test, ensure that
the Calibration Plate values are entered. See Entering the
Absorbance Plate Specifications in this chapter for more
information.
The Absorbance Test Plate or “Test Plate” can be used to conduct several
useful tests in a short time period: Mechanical Alignment, Accuracy and
Linearity, and Repeatability. The data and results for these tests can be
reviewed on the Absorbance Plate Test report. To run an Absorbance Plate
Test, you will need a Bio-Tek Absorbance Test Plate (PN 7260522) with its
accompanying data sheet.
• Mechanical Alignment: The Test Plate has precisely machined
holes to confirm the mechanical alignment of different microplate
readers. The amount of light that shines through these holes
indicates whether the reader is properly aligned. A reading of more
than 0.015 OD for any of the designated alignment wells indicates
that the light is being “clipped” and the ELx800 may be out of
alignment. If the reader fails the alignment test, review the
following possible problems and solutions:
Ensure that the Test Plate is correctly seated in the microplate
carrier, with “A1” in the upper left corner. Check the four
alignment holes to ensure that they are clear of debris.
Check the microplate carrier to ensure that it is clear of debris.
If these remedies are ineffective, call your Bio-Tek representative or
Bio-Tek Technical Support.
•
Accuracy and Linearity:
The Test Plate contains neutral density
glass filters that have assigned OD values at several wavelengths.
Since there are several filters with differing OD values, the accuracy
across a range of ODs can be established. Once it is shown that the
device is accurate at these OD values then, by definition, it is linear.
Note that there may not be a Pass/Fail indication for filter values
that are beyond the specified accuracy range of the instrument.
If the reader fails the test, review the following possible problems
and solutions:
Check the filters on the test plate to ensure they are clean. If
necessary, clean them with lens paper.
IMPORTANT! Do not remove the filters from the
test plate, and do not use alcohol or other cleaning
agents.
Bio-Tek Instruments, Inc.