Initial Preparation
1.
Plan the plate layout.
2.
Start up/warm up the Bio-Plex Multiplex Immunoassay System (
30 min
).
■
Bring diluents, including wash buffer, assay buffer, standard
diluent HB, detection antibody diluent HB, and sample diluent HB,
to room temperature (RT). Keep the other items on ice until needed
– Mix by inversion to ensure all salts are in solution
– Prepare 1x wash buffer: dilute
1 part
10x wash buffer (60 ml) with
9 parts
distilled water (540 ml)
■
Begin to thaw the frozen samples
3.
Prepare the sample dilution according to the guidelines provided in the
following table. It is important to centrifuge serum or plasma samples at
1,000 x g
for
15 min
at
4°C
to remove particulates from all samples prior to use.
Sample Type
Recommended Sample Dilution
Diluent
Serum and plasma
1:4
Sample diluent
Culture media and fluids
User defined
D 0.5% bovine
serum albumin (BSA) (w/v)
Note:
ICAM-1 and VCAM-1 require higher dilution for serum and plasma (recommended 100-fold). Refer
to the Bio-Plex Pro Human Immunotherapy Assays Instruction Manual (#10000112563) for detailed sample
preparation recommendations.
4.
Calibrate the Bio-Plex System within Bio-Plex Manager Software.
5.
Reconstitute the standards and control by adding 250 µl of standard
diluent HB to each.
Vortex
at medium speed for
5 sec
and incubate all
vials on ice for precisely
30 min
.
Bio-Plex Pro
Human Immunotherapy Panel
Quick Guide
For Use with
Instruction Manual #
Bio-Plex Pro Human Immunotherapy Assays
10000112563
This guide can be used to prepare and run a full 1 x 96-well assay plate. New
users can download the complete manual, which includes detailed instructions
and a list of kit components, at
.
