Beckman Coulter UniCel DxC 600, Instructions For Use Manual

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A13914AF
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System Description
Cartridge Chemistry: Calibration Theory
Endpoint and First-Order Chemistries
Calibration of endpoint and first-order rate chemistries involve the use of a single-level calibrator
solution or a two-level calibrator kit. Each analyte in the calibrator solution has a known
concentration value associated with it. With each new lot of calibrator solution, the values are
transferred from disk and stored in memory for later use in the calibration procedure.
For most calibrated cartridge chemistries, the system will set calibration factors based on four
calibrator replicates per calibrator level. The instrument will determine and discard the highest and
lowest of the four replicates. The remaining two values are called the usable calibrator replicates.
All four replicates will appear on the report but the average of the two usable replicates is used to
determine the calibration factor.
For other chemistries, calibration is based on two calibrator replicates per calibrator level. No
replicates are discarded. The average value of the calibrator replicates is used to determine the
calibration factor.
Endpoint and First-Order Calibration Formulas
The calibration factor is determined by using one of the following equation sets where reaction and
blank are used from the usable replicates.