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Beckman Coulter SX4400, Instructions For Use Manual

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B01198AB
October 2013

Instructions For Use

SX4400 Swinging Bucket Rotor

For Use in the Beckman Coulter
Allegra X-30 Series Centrifuges

Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA  92821  U.S.A.

Summary of Contents for SX4400

Page 1: ...B01198AB October 2013 Instructions For Use SX4400 Swinging Bucket Rotor For Use in the Beckman Coulter Allegra X 30 Series Centrifuges Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A ...

Page 2: ...c Beckman Coulter and the stylized logo are trademarks of Beckman Coulter Inc and are registered in the USPTO All other trademarks service marks products or services are trademarks or registered trademarks of their respective holders Find us on the World Wide Web at www beckmancoulter com ...

Page 3: ...hen a subsequent software version changes the information in this document a new issue will be released Initial Issue 04 2011 Rev AB 10 2013 Links to the topics that changed are listed below Chapter 1 Specifications The Rotor Buckets and Accessories Installing the Rotor Yoke Maintenance Rotor Components ...

Page 4: ...B01198AB iv Revision History ...

Page 5: ...ded could result in death or serious injury CAUTION CAUTION indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety Information for the SX4400 Rotor Hand...

Page 6: ...tained by Beckman Coulter Use of other manufacturers components or accessories in the SX4400 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Hook all four buckets loaded or empty to the rotor for every run Make sure that filled containers are loaded symmetrically...

Page 7: ...ccessories 2 Preparation and Use 5 Tubes 5 Prerun Safety Checks 5 Installing the Rotor Yoke 6 Loading the Buckets 7 Operation 9 Removal and Sample Recovery 9 Run Speeds 10 Care and Maintenance 11 Maintenance 11 Cleaning 12 Decontamination 13 Sterilization and Disinfection 14 Tube Breakage 14 Storage 14 Returning a Rotor 14 Supply List 15 Replacement Rotor Parts 15 Other 16 Beckman Coulter Inc Benc...

Page 8: ...viii Illustrations Illustrations 1 The SX4400 Rotor and Accessories 3 2 Installing the Rotor Yoke on the Centrifuge Drive Shaft 6 3 Arranging tubes in the Rotor 8 4 Seating Buckets on Yoke Pivot Pins 9 ...

Page 9: ...ix Tables Tables 1 Available Tube Adapters for the SX4400 Rotor 3 ...

Page 10: ...x Tables ...

Page 11: ...oximate deceleration time from maximum speed fully loaded 29 sec Weight of fully loaded rotor 5 7 kg 12 6 lb Rotor and bucket material anodized aluminum Bucket cover material polysulfone a The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration b Relative Centrif...

Page 12: ...e shaft during centrifugation Figure 1 shows the rotor and accessories The centrifuge identifies rotor speed during the run by means of a magnetic speed sensor system in the centrifuge rotor chamber and magnets imbedded in the rotor The overspeed system ensures that the rotor does not exceed its permitted speed Buckets and Accessories Threaded top buckets with screw on transparent covers hold seve...

Page 13: ... 4 Tie down Screw 5 Rotor Yoke Table 1 Available Tube Adapters for the SX4400 Rotor Adapters Tubes Maximum Number Tubes per Rotor Part Number pkg 2 Tubes per Adapter Description Nominal Volume N A N A Bottle polypropylene Part Number B01435 400 mL 4 392256 1 61 8 125 mm flat bottom w screw on cap a 250 mL 4 368457 1 56 5 113 mm 180 mL 4 368458 1 50 5 99 mm 125 mL 4 368459 1 44 100 mm glass 80 100 ...

Page 14: ...s open top 392257 5 17 120 mm conical 15 mL 20 368468 12 16 0 81 1 mm 10 mL 48 368465 12 17 110 mm round bottom 10 15mL 48 17 mm Monovettes 10 mL 16 100 mm Vacutainer 10 mL 16 125 mm Vacutainerb 15 mL 368467 16 13 75 mm Vacutainer 5 mL 64 Hemolyse 5 mL RIA tubes 5 mL 392263 25 12 75 mm RIA tubes 5 mL 100 368469 16 12 100 mm glass 7 mL 64 368470 16 11 38 mm 1 5 2 2 mL 64 368471 24 7 19 mm microfuge...

Page 15: ...itions using water instead of valuable samples if operating below 4 C or above room temperature Refer to Chemical Resistances publication IN 175 for information on the chemical resistances of tube and adapter materials Temperature Limits Plastic containers have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated...

Page 16: ...ee instructions under Maintenance 2 Carefully lower the yoke straight down onto the centrifuge drive shaft see Figure 2 Be sure the yoke is properly seated on the shaft Figure 2 Installing the Rotor Yoke on the Centrifuge Drive Shaft 3 Fasten the tie down screw 361367 onto the drive shaft Use the T handle rotor wrench 365636 to tighten the tie down screw firmly on the shaft CAUTION Never drop the ...

Page 17: ...rically Two factors affect symmetric loading 1 The buckets or carriers must be loaded symmetrically with respect to their pivotal axes 2 The rotor should be loaded symmetrically with respect to its center of rotation For best results load opposing buckets or carriers with the same type of labware containing the same amounts of fluid of equal density Additionally opposing buckets and their contents...

Page 18: ... the yoke by aligning the grooves in the bucket sides with the pivot pins then sliding the buckets down until the pivot pins are seated in the bucket pockets CAUTION Attach all four buckets loaded or empty to the rotor yoke If only two buckets are filled place them in opposite positions on the yoke All four positions must contain buckets during a run 4 Gently swing the buckets to ensure that they ...

Page 19: ...aped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Remove the buckets from the centrifuge 2 Remove covers if used from buckets and remove the tubes from the buckets NOTE If glass tubes break remove the glass very carefully from the adapter and bucket If all the glass particles are not contained in the bucket or adapter be careful when examining or clean...

Page 20: ...ds that exceed 4700 rpm for refrigerated centrifuges or 4200 rpm for nonrefrigerated centrifuges Speed Derating The maximum run speed listed in the rotor specifications is for operation when all conditions are within the standard specifications Maximum allowable run speed for the rotor must be reduced in some circumstances 1 The rotor can process solutions at the maximum rated speed if the solutio...

Page 21: ...rotor repair center 2 Regularly check the condition of the tie down screw 361367 if it is worn or damaged replace it Regularly check the condition of the bucket O rings 392621 if they are worn or damaged replace them 3 Check bucket covers and adapters for crazing or cracks before use Do not centrifuge cracked covers or adapters 4 Approximately once a week and after cleaning and or autoclaving lubr...

Page 22: ... damage the rotor The Rotor Cleaning Kit 339558 contains two plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water 2 Thoroughly rinse the cleaned rotor components with distilled water 3 Air dry the rotor components upside down Do not use acetone to dry the rotor 4 Lubricate the rotor pins with Spinkote 306812 Before reinst...

Page 23: ...ontaminate them using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash While Beckman Coulter has tested these materials and found that they do not damage components no guarantee of decontamination is expressed or implied Follow ...

Page 24: ... When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube sterilization and disinfection procedures Tube Breakage CAUTION To reduce the potential for corrosion clean buckets thoroughly immediately following a tube breakage If a glass tube breaks remove the gla...

Page 25: ...aminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA RMA form when mailing the rotor and or accessories Customers located outside the United States should contact their local Beckman Coulter office Supply List NOTE Publications referenced in this ma...

Page 26: ...400 Swinging Bucket Rotor Supply List Other Adapters see Table 1 Spinkote lubricant 2 oz 306812 Silicone vacuum grease 1 oz 335148 Rotor Cleaning Kit 339558 Beckman Solution 555 1 qt 339555 Rotor cleaning brush 339379 ...

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Page 29: ...tor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual This warranty is void if the r...

Page 30: ...B01198AB Warranty 2 Beckman Coulter Inc Benchtop Rotor Warranty ...

Page 31: ......

Page 32: ...iption Installation Operation Troubleshooting Care and Maintenance Available in hard copy or electronic pdf by request Additional References Chemical Resistances for Beckman Coulter Centrifugation Products IN 175 Use and Care of Tubes and Bottles IN 192 Available in hard copy or electronic pdf by request Available at www beckmancoulter com ...

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