Beckman Coulter Navios, Instructions For Use Manual, Page: 58 / 586

773232AH
3-4
Operation Principles
Light Collection, Separation And Measurement
Light Collection, Separation And Measurement
Forward Scatter Collection
The FALS (Forward Angle Light Scatter) detector collects scattered light from a particle that
intersects with a laser and delivers information roughly proportional to the size of the particle. The
forward angle light is filtered with a 488 nm band pass before it reaches the FS sensor which
generates voltage pulse signals. These signals are proportional to the amount of light the sensor
receives. As explained in
Signal Processing , the signals are processed to measure the characteristics
of the cells that scattered the light.
The forward scatter detection in the Navios flow cytometer is designed to optimize the detector for
your desired particle-size sensitivity. The Navios FS detector uses three software controlled settings
chosen for optimal versatility of a wide range of particle size characterization. The three FS angle
collection options are:
•
Wide
- used for the majority of particles between 1 micron and 20 microns. This forward angle
collection provides the best non-specific size performance blend. (1 to 19  FALS angle
collection).
•
Narrow - used for larger particle resolution <40 microns. (1 to 8  FALS angle collection).
•
Wide Enhanced
- used for sub-micron article resolution.
NOTE
For IVD applications, the wide position should be used unless stated otherwise in the application's
instructions for use.
Side Scatter and Fluorescent Light Collection
Both side scatter and fluorescence are measured 90 degrees from the laser excitation angle. Side
scatter on this system is collected opposite the fluorescence collection.
The fluorescence pickup lens filter assembly is gel-coupled to the flow cell and collects FL from the
flow cell, and focuses it.
Side Scatter
The wavelength of SS is 488 nm. It is much more intense than FL. SS is filtered with a 488 nm band-
pass (488 BP) filter that is mounted inside the fiber optic cable.