Beckman Coulter Navios, Instructions For Use Manual

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5-15
System Overview
Creating Protocols
5
Creating Protocols
1
File > New > New Protocol.
2
and Parameters button on the Cytometer Control Acquisition Setup Tab .
3
Choose the Parameters to be acquired and .
NOTE
Including TIME as a selected parameter in all protocols allows for an internal quality check on the
data acquisition. A TIME versus fluorescence plot may be helpful to monitor system fluidic and optic
conditions during acquisition of any given sample.
CAUTION
Risk of erroneous results if the parameter order is changed or parameters are
added or deleted. To prevent reporting erroneous results, verify the protocol’s
plots, regions and gates before reporting results.
4
If the parameters have changed,
a.
.
b.
Enter the desired protocol File name and .
c.
on the Cytometer Control screen.
5
Create the Plots required for acquisition. See Plots Menu .
6
If analysis is to be performed during acquisition, create the required Regions and Gates, and also
select the required statistics from the
Analysis > Select Results option. See Creating Regions and
Creating Gates .
7
File > Save Protocol As
to save the protocol.