Instrument Operation
Data Reduction
IMMAGE 800 Operations Manual A11403
System Description
September 2016
Page 2-59
2
Data Reduction
The following table describes the typical data reduction process performed by the
IMMAGE 800.
5
Chemistries which utilize AGXS testing have antigen dispensed and their
reaction monitored for an additional 20 seconds.
6
The instrument will automatically make the appropriate dilution for high
or low samples to obtain the final answer. Samples which exceed the
instrument range will be flagged as Out-of-Range Hi or Out-of-Range Lo.
Note: During the run, the system tracks all reagent volumes and can
switch to another bottle of the same lot number. If there are no additional
reagents of that lot number, the test will be set to
Pending
prior to
aspiration of sample or reagent.
Table 2.11
Sample Processing Functions
, continued
Stage
Description
(2 of 2)
Table 2.12
Data Reduction Functions
Stage
Description
1
The reaction wheel operates on a 5 second cycle time. During each cycle,
the cuvettes are spun in front of the optics station and 200 data points are
taken. These 200 points per cuvette are then calculated to a single value.
2
For each spin, the calculated value, the time the data was taken, and a
calculated quality value is logged for each cuvette.
3
When the reaction incubation time has expired on each cuvette, the data
logged for that cuvette is checked for certain mathematical bounds.
4
The data is then checked for an increasing signal, indicative of a reaction.
5
Passing the checks, the data is then mathematically curve fitted to a non-
linear function. The rate of scatter intensity is calculated from the resulting
curve.
6
The rate is directly related to the concentration.
7
A concentration test result is calculated based on the calibration factor, the
rate, and any dilution factors used.
8
Results may be scaled into alternative reporting units defined by the user.
