Beckman Coulter COULTER LH 750 System, Reference

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PN 4277248DD
3-15
OPERATION PRINCIPLES
PARAMETERS AND THEIR DERIVATION
3
Derived From WBC Histogram
Calculated
White Blood Cell (WBC) Count
WBC is the number of leukocytes measured directly, multiplied by the calibration factor. This
number is expressed as
Red Blood Cell (RBC)
RBC is the number of erythrocytes measured directly, multiplied by the calibration factor.
This number is expressed as
Hemoglobin (Hgb) Concentration
The transmittance of light (525 nm wavelength) through the lysed WBC solution in the
hemoglobin cuvette is compared to the transmittance of the same light through a reagent
blank. The system converts this ratio to an Hgb value in g/dL using a calibration factor.
Weight (mass) of hemoglobin determined from the degree of absorbance found through
photocurrent transmittance is:
Parameter Derived From
NRBC% WBC Histogram and VCS Technology
Parameter
Hct
MCH
MCHC
DIFF# parameters
RET#
IRF
MRV
NRBC#
WBC = n 103 × cells / L µ
RBC = n 106 × cells / L µ
HGB (g / dL) = Constant x log
10
Reference %T
Sample
%T