Beckman Coulter CEQ 8000, User Manual

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Consumable Items List
User’s Guide 365
Materials Required but not Supplied
Sequence
Analysis
Fragment
Analysis
Refrigerated microfuge
✔ ✔
Molecular Biology Grade: sterile dH
2
O, 95% (v/v) ethanol/dH
2
O, 70%
(v/v) ethanol/ dH
2
O
✔ ✔
Sterile tubes - 0.5 mL microfuge, 0.2 mL thin wall thermal cycling
tubes or plates with caps
✔ ✔
Thermal cycler with heated lid
✔ ✔
CEQ Sample Loading Solution (SLS)
—
✔
Mini Alpha Swab from Texwipe, P/N: TX754B (VWR)
✔ ✔
Microduster III AccTech, P/N: 58019-538 (VWR)
✔ ✔
20 mg/mL Glycogen - Boehringer Mannheim Cat. # 901 393
—
✔
3M Sodium Acetate pH5.2 - Sigma, Cat. # S7899
✔ ✔
100mM Na
2
-EDTA pH8.0 (500 mM Sigma Cat. # 7889)
✔
—
PCR enzyme and buffer
—
✔
Labeled primers (contact your local Beckman Coulter representative for
information on vendors carrying the appropriate primers)
—
✔
Thermal cycling plates and caps
—
✔