Beckman Coulter CEQ 8000, User Manual

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Fragment Analysis Procedures
278 CEQ™ 8000 Genetic Analysis System
AFLP Analysis
AFLP (Amplified Fragment Length Polymorphism) Analysis is a highly sensitive
DNA fingerprinting technique that relies on the selective amplification of restriction
fragments from a total digest of genomic DNA.
The CEQ System includes AFLP Fingerprint analysis, which allows you to rapidly
score AFLP fingerprints with output as a text format which is compatible with most
statistical and pattern matching software. The AFLP analysis tool is a cluster analysis
program that scores the presence or absence of a fragment of a given size per sample.
Using the AFLP feature
The AFLP feature takes all results from the Study and creates bins from fragments
with sizes that fall within a user-defined bin width.
For each of the possible fragments in the Study, a “1” is assigned if a fragment is found
in that sample and “0” is assigned if the fragment is absent. A table of 1’s and 0’s is
then created. The text can be exported to most other statistical analysis and
spreadsheet software.
Setting the AFLP Analysis Parameters
Open the
Analysis
menu and select the
New AFLP Analysis
command.
• This can also be accessed from the
Analyses
tab of the
Study Explorer
.
• The
New AFLP
window will be displayed.