Beckman Coulter CEQ 8000, User Manual

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134 CEQ™ 8000 Genetic Analysis System
Condition Method
The Condition method (see Figure 78) should be used before a run if the system has
not been used for a long period of time. Performing this method will fill the capillaries
with gel 6 times. Then, after separation, thoroughly purge the manifold with fresh gel
followed by a capillary fill.
Thoroughly clean the wetting tray with D.I. water before every run to remove
old or dried gel.
Creating or Editing a Method
To create and/or edit a method, perform the following steps.
1. Select
Edit | Method
from the Sample Setup Main menu.
2. Highlight the desired method in the
Choose Method to Edit
dialog box and
click
OK
.
3. In the
Method - Capillary Temperature
dialog box:
a. Enter a temperature between 35 and 60° C.
b. Select the
Wait for Cap Temp
option (if desired).
c. Click
OK
to exit the
Method
dialog box or continue with step 4 to make
additional changes to the method.
4. Select
Denature
from the
Event
list and then:
a. Enter a duration between 0 and 180 seconds (if 0 is selected, there will be no
denaturation).
b. Click
OK
to exit the
Method
dialog box or continue with step 5 to make
additional changes to the method.
3. Select
Pause
from the
Event
list and then:
a. Enter a time duration between 0 and 10 minutes.
b. Click
OK
to exit the
Method
dialog box or continue with step 6 to make
additional changes to the method.
3. Select
Inject
from the
Event
list and then:
a. Enter an injection voltage between 0.1 and 12.0 kV.
b. Enter a time duration in seconds.
c. Click
OK
to exit the
Method
dialog box or continue with step 7 to make
additional changes to the method.
4. Select
Separate
from the
Event
list, and then:
a. Enter the separation voltage between 0.1 and 12.0 kV.
b. Enter a time duration in minutes.
c. Click
Advanced
to specify the two separation phases for the method. Click
OK
to return to the
Method
dialog box.