Beckman Coulter CEQ 8000, User Manual

Page: 131 / 382

Running a Sample
User’s Guide 117
20. Select the project you just created from the
Project Name
drop-down menu and
click
OK
.
Running a Sample
Starting the Run Module
21. From the CEQ Main Menu, click on the
Run
icon and verify that the CEQ Run
window is displayed.
Installing the Capillary Array
22. Check the capillary life and usage on the life tab and if necessary, perform the
procedure “ Removing and Replacing the Capillary Array ” on page 336, and then
return here.
Installing a Gel Cartridge
23. Check the gel life and usage on the life tab and if necessary, perform the procedure
“ Removing and Replacing a Gel Cartridge/Gel Pump Plug ” on page 343, and
then return here.
Installing the Gel Waste Bottle
24. If necessary, perform the procedure “ Replacing the Gel Waste Bottle ” on page
356, and then return here.
Preparing Plates for a Run
25. Load samples into the sample plate and cover with mineral oil.
26. Load buffer (250-300 µL) into the buffer plate.
27. Fill the wetting tray with D.I. water to the level indicator marker.
No more than one 96-well plate should be processed without
replenishing the Wetting Tray.
Periodically check the liquid level in the wetting tray. Liquid level
should NEVER be allowed to rise into the eight cannula recesses of
the wetting tray lid, nor drop below the fill level indicator line. The
top surface of the wetting tray lid must remain clean and dry under
any and all circumstances.
Setting-Up and Starting the Run
27. Select
Run | Start Sample Plate
from the Run menu.
CAUTION