BD FACSymphony A3, User Manual

Page: 52 / 128

User’s Guide 52
Flushing the system
Introduction This topic describes how to perform an overall fluidics cleaning to
remove debris and contaminants from the sheath tubing, waste
tubing, and flow cell. Perform the system flush at least every
2 weeks.
Note: If you are using the FFSS, see the BD FACSFlow Supply
System User’s Guide for instructions on flushing the system.
Cautions
Procedure To perform a system flush:
1. Remove the sheath filter.
a. Press the quick-disconnects on both sides of the filter
assembly.
b. Remove the filter assembly.
c. Connect the two fluid lines.
2. Empty the sheath container and rinse it with DI water.
3. Fill the sheath container with at least 1 L of undiluted
BD FACSClean solution.
4. Empty the waste container, if needed.
5. Open the roller clamp by the fluidics interconnect, and drain
the fluid into a beaker for 5 seconds.
6. Remove the DI water tube from the SIP.
Caution: Biohazard! The cytometer hardware might be
contaminated with biohazardous material. Use 10%
bleach to decontaminate the instrument.
Caution! Do not run detergent, bleach, or ethanol
through the sheath filter. They can break down the
filter paper within the filter body, causing particles to
escape into the sheath fluid, possibly clogging the
flow cell.