BD FACSymphony A3, User Manual

Page: 43 / 128

Chapter 3: Cytometer setup 43
About the optical filters and mirrors
Introduction This topic provides a description of the optical filters and mirrors.
Filter and mirror
configurations Each PMT has an optic holder in front of it. The optic holders are
labeled with numbers indicating the wavelengths of the bandpass
filter and longpass dichroic mirror they contain (for example, 780/
60 and 750 LP, respectively). The optic holder in front of the last
PMT in the detector array contains only a bandpass filter and is
marked accordingly.
PMT
Optic holder
handle
Optic holders
The filters steer progressively shorter wavelengths of light to the
next PMT in the array as indicated by the lines and arrows on the
top of the decagon.
Optic holders,
filters, and mirrors
Optic holders house filters and mirrors. Your cytometer includes
several blank (empty) optic holders.
Caution!
To ensure data integrity, do not leave any slots
empty in a detector array when you are using the
associated laser. Always use a blank optic holder.