BD FACSymphony A3, User Manual

Page: 42 / 128

User’s Guide 42
Priming the fluidics
Introduction This topic describes how to prime the fluidics system.
When to prime the
fluidics Sometimes, air bubbles and debris may become lodged in the flow
cell. This is indicated by excessive noise in the forward and side
scatter parameters (FSC and SSC, respectively). In these cases, it is
necessary to prime the fluidics system.
Procedure To prime the fluidics:
1. Move the tube support arm to the side.
2. Remove the tube from the SIP.
3. Press the PRIME fluid control button to force the fluid out of
the flow cell and into the waste container.
Once drained, the flow cell automatically fills with sheath fluid
at a controlled rate to prevent bubble formation or
entrapment. The STANDBY button turns amber after
completion.
4. Repeat the priming procedure, if necessary.
5. Install a 12 x 75-mm tube with 1 mL of DI water on the SIP
and place the support arm under the tube. Leave the cytometer
in standby mode.
More information
• Cytometer troubleshooting (page 102)