BD FACSymphony A3, User Manual

Page: 41 / 128

Chapter 3: Cytometer setup 41
Biological
precautions
To prevent exposure to biohazardous agents:
• Put the cytometer in standby mode before disconnecting the
waste tank to avoid leakage of biohazardous waste.
• Always disconnect the waste container from the cytometer
before you empty it. Wait at least 30 seconds for pressure to
dissipate before you remove the waste cap or sensor.
• Expose waste container contents to bleach (10% of total
volume) for 30 minutes before disposal.
• Do not wet the waste tank cap. If wet, the filter in the cap will
cause the tank to malfunction. To keep the cap dry, place it on
the bench (label side up) when it is not on the tank.
Procedure To prepare the waste container:
1. Verify that the flow cytometer is in standby mode.
Press the STANDBY button on the control panel if necessary.
2. Disconnect the orange waste tubing and the black alarm
sensor line from the waste container.
Keep the lid on the waste container until you are ready to
empty it.
3. Empty the waste container.
4. Add approximately 1 L of bleach to the waste container and
close it.
5. Reconnect the orange waste tubing and make sure it is not
kinked.
6. Reconnect the black alarm sensor line.
Caution: Biohazard! Contact with biological specimens
and materials can transmit potentially fatal disease.
Caution! The waste container is heavy when full.
When emptying it, use good body mechanics to
prevent injury.