BD FACSymphony A1 Flow, User Manual

Page: 41 / 126

Do not run detergent, bleach, or ethanol through the sheath filter. They can break down the
filter paper within the filter body, causing particles to escape into the sheath fluid, possibly
clogging the flow cell.
2. Empty the sheath tank and rinse it with DI water.
3. Fill the sheath tank with at least 1 L of BD FACSClean™.
4. Empty the waste tank, if needed.
5. Open the roller clamp by the fluidics interconnect, and drain the fluid into a beaker for 5 seconds.
6. Remove the DI water tube from the SIP.
7. Prime the instrument twice:
a. Press the PRIME button on the fluidics control panel.
b. When the STANDBY button light is amber, press the PRIME button again.
8. Install a tube with 3 mL of BD FACSClean™ on the SIP and put the tube support arm underneath the tube.
See Maintenance overview (page 38) for other recommended cleaning solutions.
9. Press RUN and HIGH on the cytometer fluid control panel. Ensure that the sample fine adjust is set to 250.
Run for 30 minutes.
10. Press the STANDBY fluid control button and depressurize the sheath tank by lifting the vent valve.
11. Empty the waste tank to avoid the mixing of bleach and BD
®
Detergent Solution Concentrate.
Never mix BD
®
Detergent Solution Concentrate and bleach because they create chlorine gas.
12. Repeat steps 2 through 11 with 1.5% BD
®
Detergent Solution Concentrate.
Note: The BD
®
Detergent Solution Concentrate must be diluted before use. Mix one full 15 mL bottle of
BD
®
Detergent Solution Concentrate into 985 mL of DI water to make 1 L total.
13. Leave the system filled with 1.5% dilution of BD
®
Detergent Solution Concentrate overnight to soak (if
possible, up to 18 hours) to reduce air adhesion throughout the system. When soaking, the system power
should be disconnected and secured. Upon completion of the soak period, restore power and continue to the
next step in this procedure .
14. Repeat steps 2 through 11 with DI water.
15. Replace the sheath filter and refill the sheath tank with sheath fluid.
16. Install a tube with less than 1 mL of DI water onto the SIP.
Chapter 4 Maintenance 41