BD FACSCanto II, Reference Manual, Page: 19 / 158

Chapter 1: Introduction 19
Figure 1-2 Flow cell
Optics
The optics system in the BD FACSCanto II cytometer is composed of both
excitation optics and collection optics. Excitation optics bring light to the flow
cell. Collection optics gather the light signals emitted or scattered by the particles.
Optics Configurations
Several optics configurations are available for the BD FACSCanto II cytometer.
The 4-2 configuration can collect four fluorescence signals from a blue laser
source and two fluorescence signals from a red laser source. The 4-2-2
configuration adds a violet laser and can collect two fluorescence signals from the
violet laser source. The 5-3 configuration uses only the blue and red lasers, but
can collect an additional fluorescence signal from each laser source.
The excitation and collection optics components for the 4-2 configuration are
described in this section. The additional optics configurations and their
components are described in Appendix B, Optics Configurations.
FSC diode
where lasers
intercept
sample stream
flow cell
obscuration
bar