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1‐WELL ASSEMBLY AND CELL CULTIVATION 

 

 

 

 

 

1‐well structure bottom can be coated before culturing cells. Cells can be 
cultured in these structures by following regular protocols. 

How to prepare structures to OxyGenie™: 

1.

 

 Add in cell culture medium and other necessary substances. 

! Optimal volume for substances is 1 ml. 
2.

 

 Place the lid over the 1‐well structure. 

! Make sure it fits correctly. 
  

 

 
 
3.

 

 Place the lid lock around the 1‐well structure and the lid. 

! Make sure it grips the lid tightly and correctly around the structure. 
 

 

 

4.

 

When all these components are placed correctly, the cover can be 

added to encase the whole 1‐well structure. 

 

 

Always use new and sterile 1‐well structures. 

1‐well structures, lids and lid locks, covers and cover locks are compatible with autoclave, EtOH and 
short term UV sterilization.  

 

 

 

 

 

Summary of Contents for OxyGenie

Page 1: ...Affix Serial Number Sticker Here User Manual UM 049 OxyGenie OxyGenie...

Page 2: ...DITIONS 8 OXYGENIE OVERVIEW 9 BACKGROUND 9 HOW TO USE OXYGENIE 10 POWER SUPPLY 11 GAS BOTTLE FITTING 12 GAS BOTTLES 14 GAS BOTTLE ASSEMBLY 15 CHANGING THE GAS BOTTLE 15 GAS CYLINDER ADAPTER 16 1 WELL...

Page 3: ...TACT DETAILS 43 TABLE OF FIGURES Figure 1 Power Supply 11 Figure 2 Power display messages 11 Figure 3 Filling station to gas cylinder 13 Figure 4 Filling sttion to bottle 13 Figure 5 Control knob open...

Page 4: ...t responsibility for accidents to personnel or damage to the OxyGenie resulting from incorrect use Many unique features of the OxyGenie are covered in detail in this manual It is recommended that the...

Page 5: ...apply any shock or vibrations to the device Always place device on robust and stable surface Some parts of the device can reach high temperatures thus do not touch these parts Avoid rapid temperature...

Page 6: ...or walk in closet An accidental release of Nitrogen or Carbon Dioxide could create an asphyxiating atmosphere in a small space If the equipment is not use in a manor specified by the manufacture the p...

Page 7: ...ested refer to the DoC Certificate WEEE This equipment must be disposed of in accordance with the Waste from Electrical and Electronic Equipment WEEE Directive This product must not be treated as hous...

Page 8: ...manual in all cases where this symbol appears in order to find out the nature of the Potential Hazard and actions to be taken in order to avoid the Hazard Warning this equipment contains high voltage...

Page 9: ...nd 30 C Storage outside of this range may cause damage ENVIRONMENTAL OPERATING CONDITIONS The OxyGenie should only be operated under the following environmental conditions Temperature Between 15 C and...

Page 10: ...channel selection valve 5 Gas bottles 6 Heat plate controller and heat plate switch button 7 Device power switch battery and power display 8 Cover 9 Lid Lock 10 Lid 11 Cover Lock 12 1 well structure...

Page 11: ...lection valve and open the bottle valve Let system equilibrate Check the device Check the valves Check the heat plate Check that the device is charging Add structures to 6 well flow divider Check the...

Page 12: ...grey switch button for 3 seconds Figure 1 Power Supply Power display messages Power off Device performs quick self diagnosis Low battery put device on charge Power on Dot on bottom right indicates tha...

Page 13: ...aching filling station to it Confirm that fitting is compatible with your fill station Always wear eye protection and heavy gloves when handling high pressure gases Always point every air systems in s...

Page 14: ...YS before use because the values can change over time Make sure all the bottle attachment components are unharmed and air flow from venting hole is not blocked Attach the bottle to the filling station...

Page 15: ...d contact provider 4 1 3 2 5 6 7 8 WARNING high pressure Do not use or fill the bottle if any fractures or flaws are visible Do not apply heat Do not use excessive force when handling gas bottles Gas...

Page 16: ...can be open at a time CHANGING THE GAS BOTTLE 1 Close the bottle valve red arrow by turning the valve gently all the way to the right Wait 1 minute for pressure to settle Open the other bottle valve g...

Page 17: ...between adapter and gas cylinder regulator Attach gas cylinder adapter to device and adjust gas cylinder regulator to 1 bar Make sure that bottle valve is open and gas channel is selected correctly No...

Page 18: ...ances is 1 ml 2 Place the lid over the 1 well structure Make sure it fits correctly 3 Place the lid lock around the 1 well structure and the lid Make sure it grips the lid tightly and correctly around...

Page 19: ...ure the cover is pushed to the bottom 1 well structures are now ready to be assembled to OxyGenie Tip Use very thin layer of silicone grease in the lip of the cover 1 That will help the assembly of th...

Page 20: ...tal tubing is hid entirely Tip Few microliters of water or EtOH can be also pipetted to the OUT of covers and ensure that it bubbles to ensure gas flow If flow divider tube is not used attach it to th...

Page 21: ...te by opening locks The 6 well flow divider can be lifted and separated from the rest of the system Gas flow and temperature are maintained as long as gas is connected and flow divider is resting on t...

Page 22: ...ult temperature 37 C and when device is turned on controller starts to reach this set point Few quick tips and pictures are collected from Okolab H401 T CONTROLLER Temperature Controller User s Manual...

Page 23: ...22 How to plug in temperature sensor How to plug in USB drive...

Page 24: ...rature value Status LED Min and max temperature within time period that user can define in settings Return to homepage Heatplate options and settings Overview page Switch display mode Temperature from...

Page 25: ...e Orange indicates that the current temperature value is out of tolerance defined in the alarm settings Controller triggers alarm and will start work toward recovery from out of range value Check your...

Page 26: ...ned as None Label This menu will allow user to change the device name Press Dev1 to enter the name of the device Press save or cancel to undo WARNING do not change heating device type from device 1 Gl...

Page 27: ...ce and temperature sensor WARNING If heating ramp rate is defined in high values this may lead heating device overshoots and the temperatures can reach significantly higher value than defined set valu...

Page 28: ...emperature sensor to the bottom of the 1 well structure with commercial adhesive tape Do not cover the tip of the sensor with the tape 5 Fill the 1 well structure with water or oil Use the volume that...

Page 29: ...Type in name that can be later to be used to track down calibrations Insert accuracy that is wanted to achieve and press OK to start calibration Device allow you to follow the process Calibration is c...

Page 30: ...n be set with and and press save In the left picture temperature difference between set point 37 0 C and actual temperature 36 8 C is 0 2 C so this is the offset value After setting offset value type...

Page 31: ...That value can be used to the device by clicking yes By clicking To USB calibration data can be downloaded to USB drive By clicking Erase calibration data is deleted For this function it is possible t...

Page 32: ...erature sensor 1 Set water bath to the set point temperature used during experiments 37 C most of the time 2 Immerse temperature sensor to water bath with a certified thermometer 3 Type in the tempera...

Page 33: ...les If Day mode is chosen each day temperature data is stored in separate file If Week mode is chosen each week temperature data is stored in separate file If Month mode is chosen each month temperatu...

Page 34: ...en To save data to USB drive From subpage clicked from screwdriver icon saved data is possible to download to USB drive Connect USB drive and click To USB By clicking Erase saved data is deleted Avail...

Page 35: ...1 60 seconds 5 Choose how the data is split into files If Day mode is chosen each day temperature data is stored in separate file If Week mode is chosen each week temperature data is stored in separa...

Page 36: ...me meaning the time that system can take to reach a new set point without triggering the alarm 4 The audible alarm is enabled by ticking Buzzer The alarm is always visual To disable alarm remove tick...

Page 37: ...en sound can be disabled and sound frequency can be modified After adjustments press Save From brightness icon display brightness can be modified After adjustments press Save From calibration icon tou...

Page 38: ...s illuminated Never settings will disable top led On Alarm led will illuminate only when device is on alarm status and Always setting will allow top led to illuminate status color all the time Glance...

Page 39: ...edures Heating devices calibration procedures Temperature sensor calibration Data download Data erase To set password click password icon Flag password enable to enable password To set password click...

Page 40: ...39 Settings Summary The summary page reports technical information and summary about the Okolabs heating device settings...

Page 41: ...is pointing to the correct position 4 Check that 6 well flow divider is resting right above the heat plate 5 Check that covers are adjusted tightly around the 1 well structures 6 Check settings for he...

Page 42: ...ICULAR PURPOSE THE IMPLIED WARRANTY OF MERCHANTABILITY OR ANY IMPLIED WARRANTY ARISING OUT OF A COURSE OF DEALING OR OF PERFORMANCE CUSTOM OR USAGE OF TRADE Ruskinn Technology Limited DOES NOT WARRANT...

Page 43: ...old waste site Instead it should be taken to the appropriate collection point for the recycling of electrical and electronic equipment Alternatively please contact your local distributor for disposal...

Page 44: ...uskinn com General enquiries ruskinnoffice bakerruskinn com Website www bakerruskinn com YouTube channel http www youtube com ruskinntechnology Ruskinn Technology Limited is a registered company in Un...

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