Instruction for Use HISTO TYPE Rainbow QS6
Version: 02/2021
Page 15 of 16
11.
TROUBLESHOOTING
For interpretation trouble shooting please see the Instructions for Use for PlexTyper
®
Software.
Symptom
Possible Reason
Potential Solution
TAMRA signals
weak or absent
across whole
plate
Presence of an inhibitor in the DNA.
Try a different extraction or sample.
Insufficient DNA in the reaction.
Repeat test with correct amount of DNA.
Wrong amplification parameters.
Check PCR program.
Contaminated or degraded DNA.
Check concentration / quality of DNA.
Check DNA on a gel.
Repeat DNA isolation.
Degraded Fluorescent probes or
primers.
Avoid exposure to light and frequent thawing and
freezing.
Observe storage conditions.
Poor or no
TAMRA signal
in individual
wells
Bubbles in the reaction / residual
liquid at the inner wall of the tube.
Careful pipetting.
Spin down PCR plate.
User error.
Ensure all wells receive the required volume of
reagents.
Evaporation of the reagents due to
incorrect closing of the PCR tubes.
Make sure that the PCR tubes are closed properly.
Caution with adhesive foils in the edge area.
Strong O560 signal supressed
TAMRA signal resulting in potential
false negative allele specific results.
Use data review in PlexTyper
®
to correct results.
Signal in the
Negative
Control
Contamination with DNA or amplicon
in the negative control.
Repeat the test.
Decontaminate the workplace.
Amplification due to PCR artefact.
Review after import into PlexTyper
®
, if the signal may
be below the thresholds or the data may in fact be OK
(please note the PlexTyper
®
Instructions for Use).
12.
TRADE NAMES USED
QuantStudio™ 6 Flex System is a tradename of Applied Biosystem (Thermo Fisher Scientific)
TaqMan
®
is a tradename of Roche Molecular Systems Inc.
® Cal Fluor & Quasar Dyes are the registered trademark of LGC Biosearch Technologies
