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Instruction for Use HISTO TYPE Rainbow QS6 

Version: 02/2021 

Page 10 of 16 

 

 

 

3)  If the  excel file has not been set up in the 

QuantStudio™ 6 Flex System with the PT prefix 

number it should now be given the correct prefix obtained when the sample idenity and run 
identity was entered into PlexTyper

®

 
 

6.6 

Evaluation and interpretation of the results 

 

For  evaluation  and  interpretation  of  the  data  it  is  mandatory  to  use  the  PlexTyper

®

  software 

(available free of charge from BAG Diagnostics) in conjunction with the kit specific data files. The kit 
files  required  for  the  evaluation  are  available  from  the  BAG  Diagnostics  download  server 
(

www.service.bag-diagnostics.com

). 

 
Please  make  a  note  of  the  lot  number  of  the  kit.  The  interpretation  kit  files  are  product  and  lot 
specific. Use of incorrect kit files could result in incorrect genotyping. For interpretation of the results 
the  raw  data  must  be  transferred  from  the  thermocycler  to  a  computer  running  the  PlexTyper

®

 

software (e.g. with a suitable USB drive).  
 
 
HISTO TYPE Rainbow QS6 is a 5-channel, 96 well multiplex genotyping test. It is possible to view 
data  on  the  amplification  machine  software,  but  it  is  not  possible  to  interpret  without  use  of  the 
PlexTyper

®

  software.  For 

QuantStudio™  6  Flex  System  analysis  colour  compensation  must  be 

applied.   
 
 
 
 
 

Summary of Contents for HISTO TYPE Rainbow QS6

Page 1: ...ample information into PlexTyper Software 5 6 4 Amplification 5 6 5 Export of results from QuantStudio 6 Flex System 9 6 6 Evaluation and interpretation of the results 10 6 7 Import of the results fil...

Page 2: ...s amplified in a real time PCR with sequence specific primers SSP The primers were specially developed for the selective amplification of segments of specific HLA alleles or allele groups The amplicon...

Page 3: ...2 Real time PCR Cycler validated cycler see 4 3 Plate holder QS6 REF 726321 Variable pipettes 0 5 1000 l and pipette tips Application spatula for qPCR Seal Molecular grade DNAse free water Suitable pl...

Page 4: ...amplification detection use two separate rooms if possible Use devices and other materials only at the respective workplaces and do not exchange them 6 2 DNA Isolation The specimen material for the i...

Page 5: ...entity This Run ID should be used when setting up the file identity in the PCR machine If the PCR excel export file is not pre labelled with the RUN ID the file can be manually prefixed with the corre...

Page 6: ...te the test DNA with other concentrations For DNA of different stock concentrations use the dilution table below to adjust the DNA to working concentration Add 8 l Molecular grade water and 2 l Plex M...

Page 7: ...ely sealed particularly at the edge of the plate Make sure that the liquid has contacted the dried mix and there are no bubbles or air gaps in the reaction wells If bubbles appear gently tap the tubes...

Page 8: ...Flex System Block type Fast 96 Well 0 1mL Experiment type Comparative Ct Ct Reagent type TaqMan Reagents Run properties Standard Define Targets Please note Custom dye instrument calibration must be p...

Page 9: ...rt the raw data in excel format with the following steps 1 Select the eds file double click on a eds file If the QuantStudio RealTime PCR software is installed the software will automatically open the...

Page 10: ...s required for the evaluation are available from the BAG Diagnostics download server www service bag diagnostics com Please make a note of the lot number of the kit The interpretation kit files are pr...

Page 11: ...s possible contamination by the PlexTyper software and a warning message is generated Amplification signals above Cq 36 in the NTC are regarded as PCR artefacts and are disregarded If PCR contaminatio...

Page 12: ...No results at an HLA locus 7 WARNINGS AND DISPOSAL INSTRUCTIONS HISTO TYPE Rainbow QS6 is designed for in vitro diagnostic use The kit should only be used by specially trained qualified personnel All...

Page 13: ...t is checked for each lot with control specimens with known HLA alleles The kit determines the HLA Loci A B C DRB1 DRB3 DRB4 DRB5 DQA1 DQB1 DPA1 and DPB1 8 1 SPECIFIC PERFORMANCE CHARACTERISTICS 8 1 1...

Page 14: ...n to avoid contamination of kit reagents and other laboratory materials with amplicons or DNA The performance of a negative control without DNA in well H12 is strongly recommended No fluorescence sign...

Page 15: ...ll of the tube Careful pipetting Spin down PCR plate User error Ensure all wells receive the required volume of reagents Evaporation of the reagents due to incorrect closing of the PCR tubes Make sure...

Page 16: ...ue number RTU Ready to use 14 LITERATURE 1 Mack S J et al 2013 Tissue Antigens 81 194 203 2 Beutler E et al 1990 BioTechniques 9 166 15 REVIEW HISTORY Version 01 2021 Issue 2021 04 Initial version Ver...

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