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Axygen MAXYGENE II, Operation Manual

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DOMINIQUE DUTSCHER SAS

Summary of Contents for MAXYGENE II

Page 1: ...D O M I N I Q U E D U T S C H E R S A S...

Page 2: ...D O M I N I Q U E D U T S C H E R S A S...

Page 3: ...patent owned or controlled D O M I N I Q U E D U T S C H E R S A S...

Page 4: ...NEW USER FOLDER 13 4 3 EDITING A USER FOLDER 13 4 4 DELETING A USER FOLDER 13 5 1 RUNNING A PROTOCOL 15 5 2 PAUSE A PROTOCOL 16 5 3 VIEWING RUNNING PROTOCOL INFORMATION 16 5 4 TERMINATING A PROTOCOL 1...

Page 5: ...SPEED 28 9 8 TM CALCULATOR 29 10 1 CLEANING THE UNIT 30 10 2 CLEANING THE HEATED LID 30 10 3 REPLACING A FUSE 30 10 4 CHANGING THE THERMAL BLOCK 30 1 OPERATING TEMPERATURE ENVIRONMENT MAY BE UNSUITABL...

Page 6: ...D O M I N I Q U E D U T S C H E R S A S...

Page 7: ...the room temperature 15 30 C relative humidity 20 80 6 DO NOT block the air vents 7 Keep the side and rear of the machine at least 30cm from the wall or other machine 8 Make sure the power source con...

Page 8: ...tion that makes touchdown possible Gradient function enables optimization of annealing temperatures Preset protocols that make starting amplification easier Air intake vents on the front and sides and...

Page 9: ...ally used as number keys for the entry of time temperature password and cycle numbers When entering a user name or protocol name a small screen will pop out to display the letters after pressing the A...

Page 10: ...in the block for better contact Rotate Lid Knob in counter clockwise direction until the Lid pops up Only after the Lid pops can users slide the Lid to the rear Section of the unit in which sample tub...

Page 11: ...D panel Please use Philip head screw driver to adjust it For run summary download to computer For run summary download to computer For air output Indicate the model name serial number power specificat...

Page 12: ...older screen Pressing any key will go to User Folder screen immediately The Heated Lid heats the air in the upper part of the sample vessels at higher temperature than the reaction mixture This preven...

Page 13: ...The first method is recommended for common operations and to prevent the skin burn from the heated lid The second method is used to clean up the heating plate of the lid Rotate Lid Knob in a clockwise...

Page 14: ...D O M I N I Q U E D U T S C H E R S A S...

Page 15: ...D O M I N I Q U E D U T S C H E R S A S...

Page 16: ...l 3 a Protocol and 4 a Protocol The function map is shown in Figure 9 Various functions including Gradient can be added to the selected created Protocol Protocol Protocol Protocol Protocol User User U...

Page 17: ...t u v 8 W X Y Z w x y z 9 For the optimal performance of the MaxyGene II we recommend Axygen tubes and plates The recommended sample volume for 0 2 ml tubes or 96 well plates is 5 100ul If using the...

Page 18: ...frequently used Protocols defined by vendor while the PUBLIC folder is open to all users All Protocols are stored inside the User folders Use directional arrow keys to select a User folder Press to vi...

Page 19: ...o select a User folder Press to edit the User name A PIN is required to edit the User folder name After editing the User folder name press to save the edited User name Use directional arrow keys to se...

Page 20: ...e PIN and press or The screen will prompt Do you want to delete Press to confirm the deletion Press to abort the deletion Note User folders which contain any protocols can t be deleted except by admin...

Page 21: ...TI IM ME E L La ab b 0 07 7 2 28 8 1 11 1 0 03 3 2 25 5 3 30 0 R RU UN N N NE EW W E ED DI IT T E EX XI IT T D DE EL LE ET TE E F 1 F 2 F 3 F 4 F 5 S ST TA AR RT T M Mi ik ke e L La ab b R RU UN N 8...

Page 22: ...mperature segment Once the countdown is completed the highlighted section will continue to move to the next temperature or cycle segment and start counting down the time 2 The Lid temperature will fla...

Page 23: ...p T Ti im me e 1 1 T Tm mp p 3 3 T Tm mp p 2 25 5 C Cy yc c 2 2 T Tm mp p 1 1 C Cy yc c R Ru un n s st ta ar rt te ed d 0 08 8 2 21 1 1 11 1 1 12 2 2 20 0 5 50 0 R Ru un n w wi il ll l e en nd d 0 08...

Page 24: ...ol settings 1 Press directional arrow keys to move cursor to the temperature or time settings required to be changed 2 Enter your desired values and press or to move cursor to other values to save the...

Page 25: ...ment only the selected segment will be inserted deleted The Temperature Segment Count Temp can be modified by entering a new value 1 to 20 in the first Temperature position and pressing ENTER When the...

Page 26: ...o re enter the settings A capital letter and High temperature value will appear on the Temperature Segment graph and Low temperature value will be shown on the Temp value of the section To remove the...

Page 27: ...OP PT TI I 8 8 2 21 1 2 20 01 11 1 1 11 1 0 00 0 B Bl lo oc ck k T Te em mp p E EX XI IT T A AU UT TO O B Bl lo oc ck k T Te em mp p 1 1 50 0 A Al ll l O Of ff fs se et t i is s b ba as se e o on n B...

Page 28: ...or to re enter the values To remove the Incremental Decremental Temperature and Time settings place the cursor in the temperature segment with Incremental Decremental Temperature and Time values Sele...

Page 29: ...ill remove the Pause setting and its symbol After all settings are entered press will store the protocol Screen will Prompt Please enter protocol name A Protocol name is required to be entered B BB BB...

Page 30: ...under the original name or to as a new protocol 1 An existing Protocol can be deleted under a User s Folder 2 Under the User s Folder screen choose a Protocol Select to delete the selected Protocol 3...

Page 31: ...Users can delete unwanted Usernames and their Protocols 6 Service Mode is reserved for Service engineers 7 Ramping Speed Users can choose the High Ramping Speed or Low Ramping Speed 8 Tm Calculator P...

Page 32: ...Vo ol lu um me e 5 50 0 u ul l P Pr ro ot to oc co ol l c co om mp pl le et te ed d s su uc cc ce es ss sf fu ul ll ly y R Ru un n 1 13 3 2 20 0 1 10 0 t to o 1 14 4 2 25 5 0 00 0 E Ex xi it t P PC C...

Page 33: ...2 1 1 Y Y M M D D 2 2 M M D D Y Y 3 3 D D M M Y Y D De ef fa au ul lt t D Da at te e 0 08 8 2 21 1 1 11 1 D De ef fa au ul lt t T Ti im me e 1 11 1 0 00 0 3 33 3 P Pr re es ss s F F1 1 t to o s sa av...

Page 34: ...rm necessary maintenance and repairs Users can choose the Ramping Speed High Ramping Speed heating rate about 4 sec and cooling rate about 3 sec Low Ramping Speed heating rate about 3 sec and cooling...

Page 35: ...g gh h R Ra am mp pi in ng g S Sp pe ee ed d 2 2 L Lo ow w R Ra am mp pi in ng g S Sp pe ee ed d P Pr re es ss s F F1 1 t to o s sa av ve e y yo ou ur r s se et tt ti in ng gs s E EX XI IT T F 1 F 2 F...

Page 36: ...e tracks make sure the lid easily slides forward The fuse is located in the power socket module just below the power connector If the unit does not turn on when the power switch is turned on then repl...

Page 37: ...ement may be damaged or aged 1 Operate the unit in a environment temperature between 15 to 30 C 2 Return the unit for service Cycle time is too long 1 Operating environment temperature may be unsuitab...

Page 38: ...roblem Lid temperature exceeds 132 C Lid temperature can t reach the set temperature Reboot the unit BLOCK ERROR Block temperature sensor or heating element problems The unit can t reach the set tempe...

Page 39: ...nt Rate 4 C 3 C Gradient Temperature 30 99 C Max temp difference 24 C Max Lid Temperature 60 65 100 115 C Protocol Capacity 200 sets Operating Temperature 15 30 C Operating Humidity 20 80 RH Networkin...

Page 40: ...p 2 25 5 C Cy yc c 2 2 T Tm mp p 1 1 C Cy yc c F 1 F 2 F 3 F 4 F 5 P PR RE ES SE ET T G GR RA AD DI IE EN NT T R RU UN N 8 8 2 21 1 2 20 01 11 1 1 11 1 0 00 0 9 94 4 0 0 9 94 4 0 0 5 55 5 0 0 7 72 2...

Page 41: ...ro ol l 2 2 B Bl lo oc ck k C Co on nt tr ro ol l 0 0 5 5 F 1 F 2 F 3 F 4 F 5 P PR RE ES SE ET T T Ti im me e i in nc c R RU UN N 2 20 01 11 1 8 8 2 21 1 1 13 3 0 00 0 9 95 5 0 0 9 95 5 0 0 6 65 5 0...

Page 42: ...odels comply with the following European standards EN 61326 Group 1 Class B EN 61010 1 and EN 61010 2 010 To the best of my knowledge and belief these units conform to these standards Name Ira Augenzu...

Page 43: ...connection link 7 Select the com port COM3 8 Port settings a Bits sec 9600 b Parity None c Stop bits 1 b Flow control None 1 Place CD into computer select PL 2303 Driver on disc 2 Follow install proce...

Page 44: ...Tape TC 96 MAT 20 Sealing Mat 96 well Microplate TC96 AS 100 100 Thermal Tape PCR AS 200 100 Aluminum Sealing Tape Consumables Pack Size Description Catalog Number Compression Mat TC96 CM 10 10 Silico...

Page 45: ...well sealing Functional Temperature range 80 C to 104 C Cat TC AS 100 Silicone Compression Mat is used to seal 96 well plates when using the MaxyGene II Thermal Cycler heated lid function These silico...

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