axolotl BIOSYSTEMS axo User Manual Download Page 1 | Manualshive

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AXOLOTL BIOSYSTEMS
Industry and Trade Limited Company

User Manual

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Summary of Contents for axo

Page 1: ...AXOLOTL BIOSYSTEMS Industry and Trade Limited Company User Manual ...

Page 2: ...r researchers who work with 3D Bioprinted tissues AxoCell Stimulator is a bioreactor that can deform flexible membranes and 3D matrices in a sterile fluid environment Axolotl Biosystems focuses on providing the set of tools that makes regenative medicine and tissue engineering research easier faster and reliable Our technologies and products advance researchers in these fields and offer a set of p...

Page 3: ...are Installation Dismounting Technical Specifications General Safety Information Electrical and Environmental Information Maintenance Warranty Biomaterial Testing Functions Sine Ramp Test Blocks and Protocols Loading Specimen to Device Software Overview Origin Jog and Maximum Amplitudes Setting Up Running a Test 3 ...

Page 4: ...4 GENERAL INFORMATION 1 ...

Page 5: ... or scaffolds It has an onboard actuator and control board to enable it to run independent of a computer in an environment controlled incubator Stimulation Uniaxial Tension Cell Culture Area 6 strips up to 90mm Maximum Strain 100 Maximum Velocity 10mm s Maximum Cycle Frequency 5Hz Loading Capacity 250N Media Volume 100 385mL Weight 3100gr Cell Contacting Components Autoclavable Materials Product C...

Page 6: ...e manual reach out to Axolotl Biosystems Please use the device according to the manual Do not try to modify the parts Attention The device must be used according to its purpose and should be be used in an other way stated in this manual Any modification to the parts and wrong usage of the device will revoke warranty Reach Axolotl Biosystems about any problem you might have with your device Attenti...

Page 7: ...INFORMATION 1 7 1 4 MAINTENANCE The equipment does not require maintenance other than cleaning All parts can be cleaned with ethanol on the exterior Testing Chamber Pull Rod and Clamps can be sterilized with UV ...

Page 8: ...GETTING STARTED 2 8 ...

Page 9: ...STARTED 2 2 1 PRODUCT MAP End Clamp Specimen Clamp Power Input USB Cable 9 P o w e r B u t t o n M a i n C h a m b e r R o d L o c k T e s t C h a m b e r T e n s i o n R o d C h a m b e r L o c k E n d L o c k ...

Page 10: ... your instrument you have to install the software provided to you in an email link or USB Note that AxoCell Stimulator only works on its own software and other softwares may cause your product to malfunction and cause damage out of warranty Click on the setup software 1 2 Install software For more information Check out 2 3 Software Installation 1 Start the device using power button 3 Choose your s...

Page 11: ...stallation to complete 7 If you wish to create a desktop shortcut you can choose Create a desktop shortcut option Note that it is advised for users to do so After that you can click on Next 5 You can start the program when installation is complete 8 ...

Page 12: ...ze and clean your device you should follow steps below to dismount the device Remove the pin connecting the rod to the main chamber 3 Remove the pull rod 4 2 2 DISMOUNTING Remove the end locks 1 Remove the end clamp from the device 2 ...

Page 13: ...GETTING STARTED 2 13 Remove the chamber locks 5 Remove the chamber 6 ...

Page 14: ...BIOMATERIAL TESTING 3 14 ...

Page 15: ...ude and duration 3 1 FUNCTIONS RAMP The ramp function applies the displacement at a constant velocity Amplitude mm 2x Period s 4t Repetition Count 1 x x Position mm Period Amplitude Time s Stretch Recover Amplitude mm 2x Stretch s t Hold s t Recover s t Rest s t Repetition Count 2 x x Time s Position mm Period Amplitude Preload Endload Stretch Hold Recover Rest Phases ...

Page 16: ... Holding The deformation can be held for a given duration Recovering The recovery phase is the time during which the deformation imposed during the stretching phase is removed Resting Finally the rest phase is the time between the end of one cycle and the beginning of the next Block Block is the repeating function on a protocol Protocol Protocol is the array of test blocks Preload Endload Preload ...

Page 17: ...e bolts If you have less than 6 specimens position them close to the middle of the chamber 1 Fit the specimens into the claws and tighten the clamps using the same accessories 2 For each strip fit the specimens for every 4 bolts two in the end clamp and two in the tension rod 3 Be mindful of the jog so that tension rod has enough space to move without clashing into end clamp and other end More inf...

Page 18: ...the test Save Protocol File Ctrl S saves your file in axo format Clear Editor Ctrl C cleans the protocol and gives you a blank protocol You can exit the program with Exit Ctrl E You can use Open icon on the left and Save icon on the right under the upper tab File Tab File Tab Protocol File Editor Device Control 3 4 SOFTWARE OVERVIEW 18 ...

Page 19: ...age of the device and total estimated time of the test is shown on the protocol file editor tab Protocol File Editor is where you can add and delete protocols and change the queue Sine Block Adds a sine function block and opens the parameters tab Ramp Block Adds a ramp function block and opens the parameters tab Delete Block Deletes the selected block Moves the block up in queue Moves the block do...

Page 20: ... can use protocol file editor You can also save the protocol and load other protocols to editor from your PC Each block of the protocol is shown in the list as shown in the example You can make changes for these blocks by clicking the values Protocol Connect Device Connects selected device to software Upload Protocol Uploads the protocol shown in the software to the device Read Protocol Reads the ...

Page 21: ...hould not force the rods to clash into the end of the workspace Jog Workspace Home At home position tension rod is next to end clamp Jog Jog is the difference of tension rod and end clamp Origin Origin is the position of specimen clamps on tension rod Workspace Total space of working area BIOMATERIAL TESTING 3 3 5 ORIGIN JOG AND MAXIMUM AMPLITUDES Protocol File Editor is where you can add and dele...

Page 22: ...y works on its own software and other softwares may cause your product to malfunction and cause damage out of warranty Connect your device to your computer with USB connection provided 1 Select the device you want to connect and then click on the Connect button 2 When connection is established the log will read Device connected 3 If you want to read the protocol file that was previously uploaded t...

Page 23: ...OMATERIAL TESTING 3 After you have uploaded the protocol to device disconnect the device from your computer and place it in your incubator 7 Power off and on the device to start your testing process 8 23 ...

Page 24: ...AXOLOTL BIOSYSTEMS Industry and Trade Limited Company ...

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