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NanoPrint™ Enterprise Level LM60 and LM210

Microarray Printers

Users Manual

v.130710.1

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Summary of Contents for NanoPrint LM210

Page 1: ...NanoPrint Enterprise Level LM60 and LM210 Microarray Printers Users Manual v 130710 1 ...

Page 2: ...Copyright 1993 2013 Arrayit Corporation All rights reserved 7 10 13 2 ...

Page 3: ...served 7 10 13 3 Table of Contents Introduction page 4 System Overview page 5 Software Features page 7 System Options and Advantages page 8 System Specifications and Features page 9 System Installation and Performance page 14 Ordering Information page 22 ...

Page 4: ...array manufacturing Based on advanced nanotechnology Arrayit NanoPrint robots move with 500 nanometer resolution and deliver sub nanoliter sample volumes using high throughput patented contact printing devices Arrayit printing devices are covered by U S Patent 6 101 946 and these devices require a license for diagnostic applications Please contact us for licensing information regarding diagnostic ...

Page 5: ... of three 384 well sample microplates printing onto 60 standard glass slide substrates Worktables for printing into microplates plate size glass and other micro fluidic biochip formats are available Figure 2 Shown is the NanoPrint LM210 system with a capacity of three 384 well sample microplates printing onto 210 standard glass slide substrates ...

Page 6: ...uses superior linear drive motion control technology and proprietary Warp1 controllers and is compatible with all standard microarray surfaces made by ArrayIt and other glass substrate slide open platform vendors Systems are easily configured to print onto different types of surfaces including 96 and 384 well plates plate sized glass and proprietary cartridges and cassettes by taking advantage of ...

Page 7: ...d Validation Runtime sample and spotting views as well as a Simulation Mode Easy to use graphical Re Print Wizard The high speed high precision linear servo control system of the NanoPrint produces superior instrument performance that is essentially free of friction noise and thermal emission Combined with the compact bench top design user configurable worktable humidity and particulate control a ...

Page 8: ...assettes biochips and cartridges Worktable conveyor Microplate stackers refrigerated with lid removal and barcode reader Dehumidification Extended service warranty The value of the linear servo motor technology used on NanoPrint Microarrayers Nanometer scale positional accuracy Quiet operation No vibration No heat generation No motor dust Superior Accuracy Near maintenance free operation High spee...

Page 9: ... H cm LM60 110 x 85 x 56 cm LM210 164 x 85 x 56 cm Weight kilograms LM60 150 kg LM210 200 kg Positional resolution 500 nanometers using linear drive Warp1 control Printing speed 48 spots per second or 192 Spots second using 192 Series Pins and Printheads Destination Microarray substrates glass slides microplates custom Printing technology Arrayit Pro 946 or Stealth pins and printheads Number of pi...

Page 10: ...nication stations on the deck of the NanoPrint The ACM unit fits neatly beneath the laboratory bench or under the optional anti vibration table available with the system The ACM is extremely quiet and operates only as needed by the system Humidity is maintained in a user specified manner of 1 Wash buffers are stored in carboys in front of the ACM NanoPrint Pin Cleaning Module NanoPrint microarraye...

Page 11: ...nside the chamber assures that proper humidity levels are achieved and maintained during the entire duration of each print run Dehumidification is an option available at an extra cost and is highly recommended for users who wish to operate their systems in humid environments The humidification and dehumidification systems are triggered by sensors that automatically maintain the levels set by the u...

Page 12: ...ted microplate cooling via Peltier as an affordable and highly recommended option for users wishing to minimize sample evaporation during printing Microplate cooling is also highly recommended for protein microarray applications to minimize protein denaturation and microbial growth in recombinant protein samples The Peltier module fits directly beneath the microplate for highly efficient cooling w...

Page 13: ...e pin tool any way that they like Multiplex array processing systems plates biochips micro fluidics and cartridges can all be easily configured Superior Z Axis Resolution for Optimum Spot Morphology The Z axis encoder on the NanoPrint reads at 250 nanometer resolution Unlike belt and stepper motor driven Z axis motion control the NanoPrint offers highly precise resolution repeatability and compute...

Page 14: ...int deck is configured in a module manner allowing different worktables to be inserted and removed from the deck for different printing applications such as glass substrates microplates and proprietary cassettes and cartridges The screenshot shows a worktable allowing printing into 15 microplates 96 well for the NanoPrint LM60 ...

Page 15: ...served 7 10 13 15 Figure 4 Data showing the printing consistency of eight different SMP3B pins in a single print run using the NanoPrint LM60 The data were provided courtesy of W G Shreffler and L Bardina at the Mount Sinai School of Medicine New York USA ...

Page 16: ... Figure 5 Shown is the NanoPrint LM210 system configured in an ArrayIt class 100 cleanroom The entire deck is accessible via two doors that open and close independently for user convenience and safety The top of the Accessory Control Module ACM is shown beneath the robot ...

Page 17: ...nical replicates printing pattern worktable printing offsets speed acceleration and many other technical aspects are completely controllable by the operator via an intuitive graphical user interface GUI The NanoPrint MicroarrayManager Software allows users to write completely customized printing routines in minutes Worktables allow the user to switch between glass substrate slides and microplates ...

Page 18: ...hown is the NanoPrint 48 pin printhead designed to hold up to 48 microarray printing pins of the Professional 946 or Stealth series The attachment bayonet allows the printhead to be attached and removed for cleaning easily and precisely eliminating the need for system re calibration ...

Page 19: ...t contact between the sample layer on the pin tip and the glass printing substrate results in the formation of a printed microarray spot The NanoPrint LM60 and LM210 systems control pin movements in 500 nanometer increments for unsurpassed printing precision at speeds of 48 spots per second A total of 48 microarray printing pins of any tip size from the Professional 946 or Stealth series may be us...

Page 20: ...allows fully automated conveyor based loading and unloading of glass slide substrate microplate and custom worktables from the NanoPrint deck With a capacity of 60 worktables the WorkTable Workstation allows fully automated printing onto 1800 glass substrates 300 96 well microplates or 900 custom cassettes by special purchase quoted separately from standard NanoPrint configurations ...

Page 21: ...bstrate a popular format easily setup and executed using the NanoPrint This format is compatible with the AHC4x24 AHC1x24 and RC1x24 hybridization reaction tools Using this format and an NanoPrint LM210 Microarrayer enables the automated manufacturing of more than 5 000 microarrays per run and 1 400 using a NanoPrint LM210 ...

Page 22: ...arranty excluding pins and printheads in one year increments NPW60 12 548 NanoPrint LM210 Extended Warranty NanoPrint LM210 microarrayers include a one year warranty upon purchase Users may purchase a comprehensive Extended Warranty excluding pins and printheads in one year increments NPW210 20 423 NanoPrint Pro3 Printing Pins NanoPrint LM60 and LM210 microarrayers utilize 48 printing pins of any ...

Page 23: ... Stackers NPA Inquire NanoPrint Microplate Worktables Microplate worktables insert directly into the LM60 or LM210 deck allowing the user to print directly into 15 96 well microplates LM60 or 45 96 well microplates LM210 Worktables are easily removed to allow switching between glass substrate slides and microplates NPA Inquire NanoPrint Custom Worktables Custom microplate worktables insert directl...

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