Applied Biosystems Veriti, User Manual

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5-2 Veriti™ Thermal Cycler User Guide
Chapter 5 Utilities
Using GeneAmp
®
PCR System 9700 Run Methods
Note: This function is only available on Veriti 96-Well Thermal Cyclers.
The Veriti
™
Thermal Cycler has a Convert Method tool to guide you through
importing existing run methods from a GeneAmp® PCR System 9700 thermal
cycler.
The tool is designed to create a method for the Veriti Thermal Cycler with ramp rates
that simulate those on the 9700 thermal cycler. Although the ramp rates may be
similar in the two methods, the results on the Veriti Thermal Cycler may differ from
those from the GeneAmp PCR System 9700 and, therefore, additional optimization
may be needed.
The available conversions are specific to the type of instrument:
• Veriti 96-Well Fast Thermal Cycler – Only 9700 Max Mode run methods can
be converted.
• Veriti 96-Well Thermal Cycler – Run methods in 9600 Emulation Mode or
9700 Max Mode can be converted.
Note:
Only users who can save run methods (all users except Limited Users) have
access to this function.
To use a run method from a GeneAmp® PCR System 9700 thermal cycler:
1. Obtain a copy of the original method you want to convert, either by printing it
from the instrument or copying it manually to a piece of paper.
2. In the Main Menu screen, touch Tools Menu .
3. In the Tools Menu screen, touch
Convert a Method
to open the Convert
Method wizard:
4. Touch the check box next to the run method’s original format ( 9700 Max Mode
or 9600 Emulation Mode ) then touch (Next).