Prepare the plate
IMPORTANT!
Wear powder-free gloves and safety glasses when you prepare the
plate.
1.
Remove the ROI calibration plate from the freezer, then thaw it at room
temperature for approximately 30 minutes.
IMPORTANT!
Use the calibration plate within 2 hours of defrosting it. Until you
are ready to run the plate, store it in the dark and at ambient temperature
(15–30
℃
). Do not remove the calibration plate from its packaging until you are
ready to run it. The fluorescent dyes in the wells of the plate are photosensitive.
Prolonged exposure to light can diminish the fluorescence of the dyes.
2.
Remove the ROI plate from its packaging. Do not remove the optical film.
3.
Vortex and centrifuge the plate:
a.
Vortex the ROI calibration plate for 5 seconds.
b.
Centrifuge the plate for 2 minutes at < 1500 rpm.
IMPORTANT!
The ROI calibration plate must be well mixed and
centrifuged.
c.
Confirm that the liquid in each well of the ROI calibration plate is at the
bottom of the well. If not, centrifuge the plate again at a higher rpm and for
a longer period of time.
1.
In the QuantStudio
™
Real-Time PCR Software Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note:
If the Manage Instrument button is inactive, add your system to the My
Instruments group.
3.
In the Instrument Manager, start the calibration:
a.
Click Maintenance, then click ROI.
b.
In the ROI Calibration screen, click Start Calibration.
Perform the ROI
calibration
Chapter 2
Calibrate the instrument
ROI calibration
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
43
