1.
Prepare the matrix or sequencing standard appropriate for your application as
described in the product insert. See Appendix E, “Catalog numbers“ for catalog
numbers.
2.
Dispense the calibration chemistry into the wells specified for the plate record
you create (see “Create a spectral calibration plate“ on page 100).
3.
Seal and assemble the plate (see “Seal and assemble plates“ on page 41).
1.
In the navigation pane of the Data Collection Software, select GA
Instruments
4
ga3730
4
Protocol Manager.
1
2
1
Instrument protocols
2
Analysis protocols
2.
In the Instrument Protocols pane, click New.
3.
Select Spectral from the Run Module dropdown list.
4.
Select the dye set, polymer, and array length.
5.
Select the run module and the chemistry.
Calibration type
Run Module for the installed
capillary length
Chemistry
Matrix
Spect36_MtxStd_1
Spect50_MtxStd_POP-7
™
_1
Matrix Standard
Sequencing
Spect36_SeqStd_1
Spect50_SeqStd
Sequence Standard
Note:
Do not change settings in the Edit Parameters dialog box.
Prepare the
spectral
calibration
standard
Create a spectral
instrument
protocol
Chapter 8
Calibrate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide
99
