67
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 6
Calibration verification test
4. Press
Enter
. The GeneAmp
®
9700 automatically begins the second reading
(45 °C
setpoint). The Calibration Verification screen opens with the setpoint
value displayed.
Note:
The cover must be within
±
1 °C of 105 °C.
and
for the second reading.
6. The GeneAmp
®
9700 evaluates the calibration of the sample block temperature
for the setpoint values that you entered and displays the results. A summary
screen opens at the end of the test.
If you entered values on the Calibration Verification Test Data Sheet, compare
those values with the actual test results.
7. Press
F1
(Accept). To interpret the results, see
.
8. When you complete all measurements, be sure to:
• Press
F5
(Exit).
• Remove the 20-µL probe assembly from the sample blocks.
• Power off the digital thermometer, then clean off the oil.
• Remove the 384-well temperature verification plates from the sample
blocks.
IMPORTANT!
Make sure the sample blocks are at room temperature (~25 °C)
before removing the reaction plates.
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 45°C
Cover must be within 1°C of 105°C
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Actual temperature at 85
°
C
Actual temperature at 45
°
C
F1
F3
F4
F5
F2
Accept
Cancel
xx.x
xx.x
