6
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 1
Probe assembly
Precautions
When you use the digital thermometer:
• Make sure the probe assembly is connected to the input connector at the top of
the digital thermometer. If a probe is
not
connected when the thermometer is
powered on, the unit displays the error message “Error No Probe Found. Install
Probe after Shut Down.”
• To power on the digital thermometer, press the on/off switch on its front.
• The temperature measured by the probe assembly appears on the digital
thermometer display in degrees Celsius.
• When you complete a test, press the on/off switch to power off the digital
thermometer.
Temperature
display
differences
The sample temperature that is displayed on the thermal cycler display screen differs
from the temperature that is displayed on the digital thermometer during heating or
cooling transitions. This is because the thermal cycler displays the
calculated sample
temperature, while the digital thermometer measures and displays the
sample block
temperature. The sample temperature that is displayed by the thermal cycler is
calculated based on the ramp rate and reaction volume.
Using the probe
while running
programs
Applied Biosystems recommends that you use the probe assembly and digital
thermometer
only
for the temperature verification test and the temperature non-
uniformity test, which are described in this manual.
If you use the probe assembly and digital thermometer while running programs other
than those used in these two tests, be aware that the accuracy of the probe data
reported by the assembly
decreases
. The decrease is due to the variability introduced
into the test by placing the probe in alternate well locations and/or the selection of
temperatures other than those recommended by Applied Biosystems.
