95
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 8
Calibration verification test
5. Press
Enter
. The GeneAmp
®
9800 begins the second reading (45 °C
setpoint),
and the Calibration Verification screen opens with the setpoint value displayed.
Note:
The cover must be within
±
30 °C of 45 °C.
and
for the second reading.
The GeneAmp
®
9800 evaluates the calibration of the sample block temperature
for the setpoint values that you entered and displays the results. A summary
screen opens at the end of the test.
If you entered values on the Calibration Verification Test Data Sheet, compare
those values with the actual test results.
7. Press (
F1
) Accept. To interpret the results, see
.
8. When you complete all measurements, be sure to:
• Press
Exit
.
• Remove the 0.1-mL probe assembly from the sample blocks.
• Power off the digital thermometer, then clean off the oil.
• Remove the 0.1-mL temperature verification probe assembly from the
sample blocks.
BURN HAZARD
. Make sure the sample blocks have cooled
at least to room temperature (less than 25 °C) before removing the probe. The
blocks and probes retain heat longer than expected.
Calibration Verification
Block temp = xx.x°C Cover temp = xxx°C
Setpoint is 45°C
Cover must be within 30°C of 45°C
F1
F3
F4
F5
F2
Cancel
Calibration Verification
Actual temperature at
85 °C
Actual temperature at
45 °C
F1
F3
F4
F5
F2
Accept
Cancel
xx.x
xx.x
