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Created on 12 December 2012

C72IB006EN-A

www.anton-paar.com

Created by HJ

Page 9 of  9

Lovis 2000 M/ME Low Volume – SOP

Standard Operating Procedure for Low Sample Volume

TIP:

If this cleaning method does not yield satisfactory results, brush the short capillary with the 
supplied brush.

Cleaning the ball

TIP:

Do not handle balls with bare fingers! Finger prints influence the measurement results. If using the 
plastic tweezers also clean them to avoid contaminating a clean ball with sample residues.

1.   Put the ball into some cleaning liquid.

2.   Put the ball onto a piece of tissue paper using the plastic tweezers.

3.   Fold a bit of tissue paper over the ball. Wet it with some cleaning 

liquid. Rub the ball with the tissue paper and one finger. Wear 
protective gloves if required.

4.   Rinse the ball with some more cleaning liquid and let it dry on 

tissue paper.

Cleaning the microliter glass syringe and hollow needles

Always clean the syringe and needles to avoid contaminating one sample with another one.

TIP:

Handle the microliter syringe with care to avoid damaging this precision tool. Pull the plunger only 
as far out as the µL-scale. Do not use strongalkaline cleaning liquids that will corrode the glass. 

1.   Empty the syringe into a waste vessel.

2.   Draw in some cleaning liquid through the hollow needle and move the plunger up and down 

repeatedly. If necessary wait a bit for the cleaning liquid to mix with the sample. Empty the 
syringe into a waste vessel.

3.   Repeat the above step two to three times until no traces of sample are left. Wipe the hollow 

needle with tissue paper.

4.   If necessary, repeat the procedure with a second cleaning liquid.

5.   Remove the hollow needle.

6.   Pull the plunger up a bit and let the syringe air-dry.

7.   Dry the hollow needle with tissue paper. Blow it dry with the built-in air pump (see above, how to 

dry the short capillary, point 15., p. 8).

Brushing short capillaries

1.   Fill  some  cleaning  liquid  into the capillary (2) with a 

syringe (5) with a hollow needle (6).

2.   Push a supplied brush (12) as far as possible into the 

capillary and rotate it. Move it up and down repeatedly. 

3.   Pull the brush out again and rinse it with cleaning 

liquid. Rinse the capillary as described above.

4.   If necessary, repeat the procedure.

5.   Dry the short capillary with the built in air-pump

.

6.   Rinse the brush and wipe it with tissue paper.

TIP:

For hard-to-remove samples, soak all items in some 
cleaning liquid first.

6

5

2

12

Summary of Contents for Lovis 2000 M/ME

Page 1: ...lary dry with the built in air pump Plug a clean hollow needle 1 2 gauge 18 onto the Adapter Luer cone 13 of the air hose 2 Capillary 6 Hollow needle 13 Adapter Luer cone of air hose Insert the hollow needle into the capillary as far as possible Start the air pump with the button on the touch screen The drying time depends on the cleaning liquid on the temperature and on the ambient air humidity D...

Page 2: ...r Lovis beforehand Tap OK The available Drive is displayed Select a capillary ball combination or a list of combinations in the field Target Tap Next If there is a list select one of the combinations You can assign a new name to the combination Select Create New Capillary if no capillary ball combination data is available Tap OK Choose ball type capillary diameter with extension short and enter th...

Page 3: ...d 7 Slowly fill the sample by pushing the plunger downwards 8 While the liquid rises draw the hollow needle upwards Make sure it remains below the liquid level and below any air bubbles 9 Fill sample until the liquid reaches the tip of the capillary 10 Check the entire filling The capillary must be filled without any bubbles or particles If detecting a bubble see arrow wait till it rises to the to...

Page 4: ...hem with the hollow needle 12 Check the filling again The capillary must be filled without any bubbles or particles 13 Select a suitable clean hollow plug 7 for the capillary 2 and close it Press the hollow plug firmly into the capillary Make sure not to insert air bubbles with the plug Turn the hollow plug to insert it deeper into the capillary If necessary remove a little sample with the hollow ...

Page 5: ... filling of the capillary for bubbles and check that the Lovis stands in a stable position Refill the capillary and tap Improve Lovis suggests Apply if the Level Adjustment correction is 0 5 The Level Adjustment is valid and stored after tapping Apply 6 Adjusting Lovis Measuring Systems Manager and Administrator only TIP If you have ordered a factory adjustment import the adjustment from the USB f...

Page 6: ...ion between adjustment and verification default 0 5 7 Fill the capillary once again with your standard liquid Do NOT change the ball Follow the instructions on the display When the verification is finished the runtime deviations for each adjusted angle are displayed TIP After a verification the runtime deviations to the values of the preceding original adjustment are displayed Typically these valu...

Page 7: ...surement is successfully completed if the progress bar turns green and the message above reads Finished The Lovis Condition is valid Menu Item Lovis Module Parameters Default Value Temperature Settings Temperature Measuring Temperature 20 C Temperature Equilibration This is the time Lovis waits for the sample to reach the measuring temperature after starting measurement and after the capillary blo...

Page 8: ...mple 7 Move the plunger up and down rapidly to improve the cleaning effect Draw the cleaning liquid back into the syringe Empty the syringe into a waste vessel 8 Repeat the two above steps two to three times until no traces of sample are left in the capillary If the ball is still inside let it roll out and rinse the capillary without the ball once 9 If necessary repeat the cleaning procedure with ...

Page 9: ...r only as far out as the µL scale Do not use strongalkaline cleaning liquids that will corrode the glass 1 Empty the syringe into a waste vessel 2 Draw in some cleaning liquid through the hollow needle and move the plunger up and down repeatedly If necessary wait a bit for the cleaning liquid to mix with the sample Empty the syringe into a waste vessel 3 Repeat the above step two to three times un...

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